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GE17-5280-01

nProtein A Sepharose™ 4 Fast Flow

Cytiva 17-5280-01, pack of 5 mL

Synonyma:

Sepharose 4 Fast Flow

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About This Item

UNSPSC Code:
41106500
NACRES:
NA.56

ligand

native protein A (S. aureus)

packaging

pack of 5 mL

manufacturer/tradename

Cytiva 17-5280-01

storage condition

(20% Ehtanol)

matrix

4% cross-linked agarose

average diameter

90 μm (d50v)

cleaning

2-10

working range

3-9

capacity

>30 mg binding capacity(human IgG/ml)

suitability

suitable for bioprocess medium

storage temp.

2-8°C

Související kategorie

General description

nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to Sepharose™ 4 Fast Flow for the purification of monoclonal and polyclonal antibodies at both laboratory and process scale.

nProtein A Sepharose™ 4 Fast Flow is native protein A coupled to the well established Sepharose™ 4 Fast Flow base matrix. The native protein A ligand is produced by fermenting a selected strain of Staphylococcus aureus. The purified protein is coupled to the cross-linked 4% agarose base matrix by the cyanogen bromide technique, giving a highly stable medium with minimal non-specific adsorption. nProtein A Sepharose™ 4 Fast Flow is manufactured without using animal-derived components.

nProtein A Sepharose™ 4 Fast Flow has nearly twice the total IgG binding capacity of Protein A Sepharose™ CL-4B, and is suitable for recovery and purification of monoclonal antibodies from cell culture at both laboratory and process scale. nProtein A Sepharose™ 4 Fast Flow was developed and tested in co-operation with leading manufacturers of purified monoclonal antibody products, and is used in routine commercial production.

As member of the BioProcess media range, nProtein A Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Features and Benefits

  • Replaces Protein A Sepharose™ 4 Fast Flow, the first Cytiva Protein A medium for large-scale purification of antibodies.
  • Used in routine commercial production of monoclonal antibodies
  • Free from animal-derived components.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Storage Class

3 - Flammable liquids

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Osvědčení o analýze (COA)

Lot/Batch Number

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Sortimentní položky

Purification Using Protein A-based Chromatography Media

This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.

Purifikace pomocí chromatografických médií na bázi proteinu A

Tato stránka ukazuje různé možnosti purifikace pro chromatografická média se sefarosou proteinu A a popisuje typické vazebné a eluční podmínky pro chromatografická média se sefarosou proteinu A.

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Protokoly

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from Cytiva.

Testy vytahováním

Tato stránka poskytuje informace o různých pull-down testech pro další izolaci multiproteinových komplexů za účelem identifikace jejich složek pomocí produktů společnosti Cytiva.

Protein A-Based Separation with Cytiva Products

Protein A, coupled to Sepharose, binds IgG via its Fc regions, facilitating affinity chromatography purification.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Zobrazit všechny

Související obsah

Protein Pull-Down Techniques

Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.

Techniky vytahování bílkovin

Zkoumejte interakce protein-protein in vitro pomocí pull-down testů s využitím afinitních metod, GST pull-down, TAP a koimunoprecipitace.

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.