E8008

Ethylenediaminetetraacetic acid solution

0.02% in DPBS (0.5 mM), sterile-filtered, BioReagent, suitable for cell culture

• Synonyma: EDTA solution
• Empirický vzorec (Hillův zápis): C₁₀H₁₆N₂O₈
• Číslo CAS: 60-00-4
• Molekulová hmotnost: 292.24
• NACRES: NA.75
• PubChem Substance ID: 24894664
• eCl@ss: 39030907
• UNSPSC Code: 12352204
• MDL number: MFCD00003541
• Beilstein/REAXYS Number: 1716295

Vlastnosti

• sterility: sterile-filtered
• Quality Segment: 200
• product line: BioReagent
• form: solution
• concentration: 0.02% in DPBS (0.5 mM)
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: 7.1-7.7
• shipped in: wet ice
• storage temp.: 2-8°C
• SMILES string: OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20)
• InChI key: KCXVZYZYPLLWCC-UHFFFAOYSA-N

Popis

General description

Ethylenediaminetetraacetic acid (EDTA) is an aminopolycarboxylic acid and a hexadentate ligand. It chelates with metal ions to form an octahedral complex especially with cations. Ethylenediaminetetraacetic acid (EDTA) is an anticoagulant, which prevents clotting of blood. It is implicated in platelet aggregation in pseudothrombocytopenia. EDTA chelates with calcium and is effective in solubilisation of the atheromatous plaques. Calcium disodium EDTA administration is effective in slowing down the progression of chronic kidney disease. It is a chelator used to remove calcium from cell washing and suspension media. EDTA reduces cell clumping.

Application

Ethylenediaminetetraacetic acid solution has been used to remove confluent cell monolayers in swine testicular (ST-cells) culture. It has also been used to detach embryonic stem cells (ESCs) from matrigel and primary human fibroblasts.

Physical form

Solution in Dulbecco′s phosphate buffered saline without calcium and magnesium

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable