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D1152

Sigma-Aldrich

Dulbecco′s Modified Eagle′s Medium - high glucose

HEPES Modification, With 4500 mg/L glucose, L-glutamine, and 25 mM HEPES, without sodium bicarbonate and pyruvate, powder, suitable for cell culture

Synonyma:

DME, DMEM

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: no
NaHCO3: no
phenol red: yes
HEPES: 25 mM
L-glutamine: yes
glucose: high

shipped in

ambient

storage temp.

2-8°C

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Související kategorie

Application

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

Quantity

Formulated to contain 17.3 grams of powder per liter of medium.

Reconstitution

Supplement with 3.7 g/L sodium bicarbonate.

also commonly purchased with this product

Č. produktu
Popis
Stanovení ceny

supplement

Č. produktu
Popis
Stanovení ceny

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Protokoly

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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