P19 Cell Line from mouse

95102107, mouse embryo, Not specified

• UNSPSC Code: 41106514

Vlastnosti

• product name: P19 Cell Line from mouse, 95102107
• biological source: mouse embryo
• description: Mouse teratocarcinoma
• growth mode: Adherent
• karyotype: Euploid
• morphology: Not specified
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Mouse teratocarcinoma

Cell Line Description

The P19 cell line was derived from an embryonal carcinoma induced in a C3H/He strain mouse. The cells can be cloned at high efficiency in medium containing 0.1mM β-mercaptoethanol. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only.

Application

Differentiation studies

Culture Medium

α MEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2.5% Foetal Bovine Serum (FBS) + 7.5% Calf Serum (CS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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