CHO-K1/SF

93061607, hamster ovary, Epithelial

• UNSPSC Code: 41106514

Vlastnosti

• product name: CHO-K1/SF, 93061607
• biological source: hamster ovary
• growth mode: Adherent
• karyotype: Not specified
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

Cell Line Origin

Hamster Chinese ovary (MEM adapted)

Cell Line Description

This MEM adapted sub-line of CHO-KI is used in Pertussis Toxin research and for quality control of batch production.

Application

CHO-K1/SF has been used to sequence the microRNA transcriptome and to identify, annotate and profile microRNAs as targets for cellular engineering. It has also been used to determine the pertussis toxin (PT) content of purified Bordetella pertussis fimbriae.

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Other Notes

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