Vero C1008 [Vero 76, clone E6, Vero E6]

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 85020206, African green monkey kidney, Epithelial

• Synonyma: Vero 76 Clone E6 Cells, Vero Cells, Vero E6 Cells, VeroC1008 Cells
• UNSPSC Code: 41106514

Vlastnosti

• biological source: African green monkey kidney
• description: Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)
• packaging: tube of 5 μg 85020206-DNA-5UG; pkg of vial of cells 85020206-1VL
• growth mode: Adherent
• karyotype: 2n = 60
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Popis

General description

Vero cells are derived from the kidney of an African green monkey. These are anchorage-dependent cells that have applications in molecular and cell biology research. Vero E6 cells enable achieving high titers of severe acute respiratory syndrome coronavirus (SARS-CoV virus) and are also used in the cultivation of Rabies virus, Reovirus and Japanese encephalitis virus.

Cell Line Origin

Monkey African Green kidney

Cell Line Description

A subclone of Vero76 (Sigma catalog no. 85020205) exhibiting a similar range of virus susceptibility (haemorrhagic viruses). They show some degree of contact inhibition and are suitable for supporting the growth of slowly replicating viruses.

Application

VERO C1008 has been used for transfection, cultivation and propagation of West Nile virus, yellow fever virus, ZIKA, Ebola virus, Sabethes flavivirus (SbFV), Crimean-Congo Haemorrhagic Fever (CCHF), Human-derived Lake Victoria Marburg virus strain Popp.

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:10, i.e., seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Other Notes

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