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A3422

Sigma-Aldrich

Achromopeptidase from bacteria

partially purified powder, ≥20,000 units/mg solid

Synonyma:

ACP, Achromopeptidase

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About This Item

Číslo enzymu podle klasifikace EK:
3.4.21.50 (BRENDA, IUBMB)
MDL number:
MFCD00130333
UNSPSC Code:
12352204
NACRES:
NA.54

form

partially purified powder

Quality Level

200

specific activity

≥20,000 units/mg solid

mol wt

27 kDa

foreign activity

Collagenase, present

storage temp.

−20°C

Application

Achromopeptidase from bacteria may be used:
  • in the homogenization step for genomic DNA extraction from microbial biofilm
  • in the lysis of gram-positive strains(
  • for DNA extraction from Nocardia strains

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Biochem/physiol Actions

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.

Unit Definition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Physical form

Partially purified powder.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Osvědčení o analýze (COA)

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Protokoly

Enzymatic Assay of Achromopeptidase

To measure achromopeptidase activity, this procedure uses bacterial cells and a turbidimetric rate assay. Turbidity is measured at 600 nm using a spectrophotometer.

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