A1009
Albumin methylated from bovine serum
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
Doporučené produkty
biological source
bovine
Quality Level
form
powder
origin
USA origin
technique(s)
affinity chromatography: suitable
fractionation: suitable
impurities
≤10% water (Karl Fischer)
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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Application
Albumin methylated from bovine serum (mBSA) has been used as a coating for immunoplates in enzyme-linked immunosorbent assay (ELISA) of anti-dsDNA IgG antibodies. It has also been used to induce arthritis in mice models.
As an affinity ligand on diatomaceous earth support (MAK chromatography), a medium for fractionation of different forms of DNA and for separating aminoacyl-tRNAs. Different modes of binding of double-stranded, denatured, and replicating forms of DNA account for the efficacy of the method.
Biochem/physiol Actions
Albumin is a major circulating component of the plasma and is a highly soluble multifunctional protein. It plays a role in several physiological and pharmacological processes. Albumin binds to metals, cholesterol, hormones, bile pigments, drugs, and fatty acids to facilitate their transport. It regulates osmotic pressure and distributes fluids between various compartments.
Preparation Note
Methyl ester of albumin prepared per Mandell, J.D. and Hershey, A.D., Anal. Biochem., 1, 66 (1960).
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Proceedings of the National Academy of Sciences of the United States of America, 48, 1454-1461 (1962-08-01)
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