90819

Bis(2,2′-bipyridine)-(5-aminophenanthroline)ruthenium bis(hexafluorophosphate)

BioReagent, suitable for fluorescence

• Synonyma: Ru(bpy)2(phen-5-NH₂)(PF₆)₂
• Empirický vzorec (Hillův zápis): C₃₂H₂₅N₇Ru · 2 PF₆
• Číslo CAS: 84537-86-0
• Molekulová hmotnost: 898.59
• MDL number: MFCD20921517
• UNSPSC Code: 12171500
• PubChem Substance ID: 57653411
• NACRES: NA.21

Vlastnosti

• product line: BioReagent
• form: powder
• reaction suitability: core: ruthenium; reaction type: Photocatalysis; reagent type: catalyst
• fluorescence: λ_ex 458 nm; λ_em 612 nm in 0.1 M Tris pH 7.0
• suitability: suitable for fluorescence
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: −20°C
• SMILES string: [Ru++].F[P-](F)(F)(F)(F)F.F[P-](F)(F)(F)(F)F.c1ccc(nc1)-c2ccccn2.c3ccc(nc3)-c4ccccn4.Nc5cc6cccnc6c7ncccc57
• InChI: 1S/C12H9N3.2C10H8N2.2F6P.Ru/c13-10-7-8-3-1-5-14-11(8)12-9(10)4-2-6-15-12;2*1-3-7-11-9(5-1)10-6-2-4-8-12-10;2*1-7(2,3,4,5)6;/h1-7H,13H2;2*1-8H;;;/q;;;2*-1;+2
• InChI key: QQMMCXKCYXQNNA-UHFFFAOYSA-N

Popis

Application

Used for photoinduced intramolecular electron transfer in peptide-bridged molecules.

Features and Benefits

Sigma-Aldrich offers a series of reactive dyes based on ruthenium complexes for use in fluorescence polarization assays and fluorescence lifetime imaging applications. They have been designed specifically to have long fluorescence lifetimes. These dyes were first synthesized in the labs of Dr. Joseph Lakowicz at the Center for Fluorescence Microscopy. These laser-excitable metal ligand complexes have fluorescence lifetimes near 500 nanoseconds.
These dyes can be used to label amines on biomolecules under mild conditions. Advantages of these dyes as protein labels include high photostability, good water solubility, a lack of dye-dye interactions and large Stokes′ shifts.
In addition, the fluorescence signal of long-lived fluorophores can be gated to eliminate the emission from short-lifetime fluorophores and autofluorescence from cells and biomolecules to further improve sensitivity.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Other Notes

Photoinduced intramolecular electron transfer in peptide-bridged molecules

Bezpečnostní informace

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)