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87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

suitable for fluorescence, ≥95.0% (HPLC)

Synonyma:

5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

Přihlásit pro zobrazení organizačních a smluvních cen.

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Vám/Skladová položkaDostupnostCena
5 mg
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
7 720,00 Kč
5 404,00 Kč

O této položce

Empirický vzorec (Hillův zápis):
C29H19NO11
Číslo CAS:
150206-05-6
Molekulová hmotnost:
557.46
NACRES:
NA.32
PubChem Substance ID:
57653248
UNSPSC Code:
12352108
MDL number:
MFCD00037466

5 404,00 Kč

Ceníková cena7 720,00 KčUložit 30 %
Obraťte se na zákaznický servis a vyžádejte si informaci o dostupnosti.
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Quality Segment

100

assay

≥95.0% (HPLC)

form

powder

solubility

DMF: soluble, DMSO: soluble

fluorescence

λex 492 nm; λem 517 nm

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

CC(=O)Oc1ccc2c(Oc3cc(OC(C)=O)ccc3C24OC(=O)c5cc(ccc45)C(=O)ON6C(=O)CCC6=O)c1.CC(=O)Oc7ccc8c(Oc9cc(OC(C)=O)ccc9C8%10OC(=O)c%11ccc(cc%10%11)C(=O)ON%12C(=O)CCC%12=O)c7

InChI

1S/2C29H19NO11/c1-14(31)37-17-4-7-21-23(12-17)39-24-13-18(38-15(2)32)5-8-22(24)29(21)20-6-3-16(11-19(20)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34;1-14(31)37-17-4-7-20-23(12-17)39-24-13-18(38-15(2)32)5-8-21(24)29(20)22-11-16(3-6-19(22)28(36)40-29)27(35)41-30-25(33)9-10-26(30)34/h2*3-8,11-13H,9-10H2,1-2H3

InChI key

LZFDYQHOQDFMOA-UHFFFAOYSA-N

Other Notes

Continuous determination of the intracellular pH in bacteria by fluorometry[1]; labeling human intervertebral disk cells in vitro for fluorescence microscopy[2]

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Tato položka
C50412187946940
5-Carboxy-fluorescein diacetate N-succinimidyl ester suitable for fluorescence, ≥95.0% (HPLC)

Sigma-Aldrich

87444

5-Carboxy-fluorescein diacetate N-succinimidyl ester

6-Carboxyfluorescein diacetate ≥95% (HPLC)

Sigma-Aldrich

C5041

6-Carboxyfluorescein diacetate

5(6)-Carboxyfluorescein diacetate BioReagent, suitable for fluorescence, ≥90.0% (HPLC)

Sigma-Aldrich

21879

5(6)-Carboxyfluorescein diacetate

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester suitable for fluorescence, ≥75% (HPLC)

Sigma-Aldrich

46940

6-[Fluorescein-5(6)-carboxamido]hexanoic acid N-hydroxysuccinimide ester

suitability

suitable for fluorescence

suitability

-

suitability

suitable for fluorescence

suitability

suitable for fluorescence

fluorescence

λex 492 nm; λem 517 nm

fluorescence

-

fluorescence

λex 492 nm; λem 517 nm in 0.1 M Tris pH 8.0 (esterase)

fluorescence

λex 490 nm; λem 514 nm (pH 8.2), λex 491 nm; λem 515 nm in 0.1 M Tris pH 9.0

solubility

DMF: soluble, DMSO: soluble

solubility

chloroform/ethanol (1:1): 9.80-10.20 mg/mL, clear, colorless to light yellow

solubility

DMSO: soluble

solubility

DMF: soluble, methanol: soluble

form

powder

form

powder

form

powder

form

solid

assay

≥95.0% (HPLC)

assay

≥95% (HPLC)

assay

≥90.0% (HPLC)

assay

≥75% (HPLC)

Quality Level

100

Quality Level

100, 200

Quality Level

100

Quality Level

100

Skladovací třída

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Osvědčení o analýze (COA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  

  1. Can user the product 5-Carboxy-fluorescein diacetate N-succinimidyl ester 87444 in cells living infected with parasite?

    1 answer

    1. This application will need to be validated by the end user. This product is passively taken up by cells. Fluorescence occurs when the dye is hydrolyzed by esterases within the cell. The dye reacts covalently with amine groups present on intercellular proteins, which allows for long-term staining effects. This dye may be taken up by the parasite, as well.

      The link below may offer additional helpful information:
      https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/carboxyfluorescein-diacetate-succinimidyl-ester

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. Can product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, be used in flow cytometry?

    1 answer

    1. Yes, product 87444, 5-Carboxy-fluorescein diacetate N-succinimidyl ester, can be used in flow cytometry. The optimal concentration range for cell labeling is 2.5 uM to10 M. The concentration of the cell line will have to be optimized.

      Helpful?

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