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10072802

CCB cell line from carp

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen


About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

fish brain

packaging

tube of 5 μg 10072802-DNA-5UG
pkg of vial of cells 10072802-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Squamous

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

Cell Line Origin

Common carp brain cell line, koi herpesvirus isolation

Cell Line Description

The CCB cell line was developed from Common carp (Cyprinus carpio carpio) brain.

Packaging

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Culture Medium

EMEM (EBSS) + 2 mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Fetal Bovine Serum (FBS). 10 mM HEPES can be included if a CO2 incubator is not available.

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 to 1:6, i.e., seeding at 2 x 104 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 25 °C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Irene Cano et al.
International journal of molecular sciences, 21(22) (2020-11-15)
Seroconversion and the mucosal lysozyme G (lysG), complement 3 (c3), and immunoglobulins M (IgMsec) and Z2 (IgZ2) were measured for up to 900 degree days (DD) in skin swabs from common carp exposed to koi herpesvirus (KHV or CyHV-3) at
Irene Cano et al.
Animals : an open access journal from MDPI, 11(2) (2021-02-13)
Fluorescence real-time LAMP assays were designed for the orf43 gene of CyHV-3 European genotype and the p4a gene of the CEV genogroup I. A third LAMP assay to detect the ef1a gene of the host common carp was designed as
Agnieszka Troszok et al.
Journal of fish diseases, 41(11), 1709-1718 (2018-08-26)
Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus (KHV), is an aetiological agent of a virulent and lethal disease in common and koi carp. In this study, we examined in vitro the anti-CyHV-3 activity of acyclovir (ACV), nucleoside analogue commonly

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