PHR1428
HEPES
certified reference material,pharmaceutical secondary standard
Synonyma:
HEPES, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
About This Item
Doporučené produkty
product name
HEPES, Pharmaceutical Secondary Standard; Certified Reference Material
grade
certified reference material
pharmaceutical secondary standard
Quality Level
API family
hepes
CofA
current certificate can be downloaded
technique(s)
HPLC: suitable
gas chromatography (GC): suitable
pH
5.0-6.5 (25 °C, 238 g/L)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
application(s)
pharmaceutical (small molecule)
format
neat
storage temp.
2-30°C
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
Inchi Key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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General description
Pharmaceutical secondary standards for application in quality control, provide pharma laboratories and manufacturers with a convenient and cost-effective alternative to the preparation of in-house working standards.
Application
- HEPES has been used in a wide variety of applications, including tissue culture.
- It is used to buffer cell culture media in air.
- Finds its usage in invitro experiments on Mg.
- Recognized as one of Dr.Good′s recommended buffers, used in some cell culture media as a buffering agent and also in various biochemical reactions.
- Recently, in the production of radiopharmaceuticals, it is the buffer of choice for scientific labeling.
HEPES may also be used as:
- Component of homogenization buffer to homogenize adipose tissue for liquid chromatography/mass spectrometry and in HN buffer for dissolving viral stock titer after dot blot hybridization.
- Pharmaceutical reference standard for the determination of the analyte in bulk drug and pharmaceutical formulations by high performance liquid chromatography.
Analysis Note
Other Notes
Footnote
Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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