DIUTPS-RO

Digoxigenin-11-dUTP, alkali-stable

• Synonyma: Digoxigenin-11-dUTP, alkali-labile
• UNSPSC Code: 41105500
• NACRES: NA.54
• Assay: ≥85% (HPLC)
• Form: solution
• Storage temp.: −20°C

Vlastnosti

• assay: ≥85% (HPLC)
• form: solution
• mol wt: M_r 1090.7
• packaging: pkg of 125 μL (11558706910 [1 mM]), pkg of 5 × 125 μL (11570013910 [5x1 mM]), pkg of 25 μL (11093088910 [1 mM])
• manufacturer/tradename: Roche
• λmax: 222 and 292 nm, 22430 and 8780
• storage temp.: −20°C

Popis

General description

Digoxigenin-11-dUTP, alkali-stable is provided in 1 mM tetralithium salt, solution.

Application

Digoxigenin-11-dUTP, alkali-stable has been used to label probes for
fluorescent in situ hHybridization (FISH)[1] and polymerase chain reaction (PCR)[2]
Note: When this nucleotide is subjected to 0.1 - 0.5 NaOH at +15 to +25°C, the DIG moiety will remain attached. It is therefore ideal for use in DIG-labeled DNA that needs to survive exposure to alkaline treatment. However, if you intend to make a DIG-labeled probe that can be removed by alkali denaturation (e.g ., during stripping and reprobing of blots), do not use this alkali-stable preparation. Instead, use the alkali-labile form of DIG-11-dUTP.

Biochem/physiol Actions

Digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) replaces deoxythymidine triphosphate (dTTP) in the random-primed DNA labeling reaction or in nick translation in a ratio of 35% DIG-11-dUTP and 65% dTTP. DIG-11-dUTP can also replace dTTP in labeling during polymerase chain reaction (PCR) (e.g., with Taq DNA Polymerase), 3′-tailing (with terminal transferase) and cDNA synthesis (e.g., with transcriptor or M-MuLV reverse transcriptase). It is ideal as a substrate for DNA polymerase, Taq DNA polymerase, terminal transferase and reverse transcriptase.

Features and Benefits

Digoxigenin is bound to deoxyuridine triphosphate via an alkali-resistant ether linkage.

Resistance to alkali: The preparation is stable in the presence of 0.1 - 0.5M NaOH at +15 to +25°C.

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).
Storage conditions (working solution): A decomposition of approx. 5% may occur within 4 weeks at 15 to 25 °C.

Analysis Note

Typical analysis: At least 85% DIG-11-dUTP (HPLC, area%).
Function tested by RPL.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: does not flash
• flash_point_c: does not flash