11681460001
Roche
INT/BCIP Stock Solution
solution, pkg of 3 mL
Synonyma:
5-bromo-4-chloro-3-indoyl phosphate, BCIP
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352204
Doporučené produkty
form
solution
Quality Level
mol wt
(INT: Mr = 505.7; BCIP toluidine salt: Mr = 433.6)
packaging
pkg of 3 mL
manufacturer/tradename
Roche
storage temp.
2-8°C
General description
BCIP (5-bromo-4-chloro-3-indoyl phosphate) is the AP (alkaline phosphatase)-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT (iodonitrotetrazolium) is the oxidant producing a red dye. The reaction product has a reddish-brown color and is insoluble in water.
Application
INT/BCIP Stock Solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, that includes:
- Southern blot
- Western blot
- immunohistochemistry and immunocytochemistry
Specifications
Formulas: INT: C19H13CIN5O2; BCIP: C8H6NO4BrCIP x C7H9N
Principle
BCIP is the AP-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT is the oxidant producing a red dye.
Physical form
Solution of 33 mg/ml INT (2-[4-iodophenyl]-3-[4-nitrophenyl]-5-phenyltetrazolium chloride) and 33 mg/ml BCIP (5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in DMSO)
Preparation Note
Working solution: Preparation of 10 ml Staining Solution
Bring the stock solution to 15 to 25 °C until all components are dissolved.
Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl2, 0.1 M NaCl.
Note: Prepare the staining solution shortly before use.
Preparation of Additional Solutions Required
Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven.
Information Note: The solution remains turbid.
Bring the stock solution to 15 to 25 °C until all components are dissolved.
Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl2, 0.1 M NaCl.
Note: Prepare the staining solution shortly before use.
Preparation of Additional Solutions Required
Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven.
Information Note: The solution remains turbid.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Zákazníci si také prohlíželi
Rahul Satija et al.
Nature biotechnology, 33(5), 495-502 (2015-04-14)
Spatial localization is a key determinant of cellular fate and behavior, but methods for spatially resolved, transcriptome-wide gene expression profiling across complex tissues are lacking. RNA staining methods assay only a small number of transcripts, whereas single-cell RNA-seq, which measures
Practical Methods in Cardiovascular Research
Dhein S
Springer Science & Business Media (2006)
Peichao Li et al.
Carcinogenesis, 42(1), 136-147 (2020-07-28)
Hexavalent chromium [Cr(VI)] is a potent human lung carcinogen. Multiple mechanisms have been proposed that contribute to Cr(VI)-induced lung carcinogenesis including oxidative stress, DNA damage, genomic instability and epigenetic modulation. However, the molecular mechanisms and pathways mediating Cr(VI) carcinogenicity have
Anna-Carina Weiss et al.
Development (Cambridge, England), 141(17), 3420-3430 (2014-08-21)
The vesico-ureteric junction (VUJ) forms through a complex developmental program that connects the primordium of the upper urinary tract [the nephric duct (ND)] with that of the lower urinary tract (the cloaca). The signals that orchestrate the various tissue interactions
Federico Tessadori et al.
eLife, 10 (2021-08-11)
Organ laterality refers to the left-right asymmetry in disposition and conformation of internal organs and is established during embryogenesis. The heart is the first organ to display visible left-right asymmetries through its left-sided positioning and rightward looping. Here, we present
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
Obraťte se na technický servis.