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11426338910

Roche

Anti-Fluorescein-AP, Fab fragments

from sheep

Synonyma:

antibody

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
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About This Item

UNSPSC Code:
12352203

biological source

sheep

Quality Level

100

conjugate

alkaline phosphatase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

primary antibodies

clone

polyclonal

form

solution

packaging

pkg of 150 U (200 μl)

manufacturer/tradename

Roche

isotype

IgG

storage temp.

2-8°C

General description

Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)

Specificity

The polyclonal antibody reacts with free and bound fluorescein.

Application

Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Southern blot
  • Western blot
  • Electrochemical biosensing technique for the detection of 16S rRNA

Biochem/physiol Actions

Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).

Features and Benefits

Contents
Solution, stabilized

Preparation Note

  • Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
  • ELISA: 150 to 300mU/ml
  • Immunohistocytochemistry: 250 to 500mU/ml
  • In situ hybridization: 1.5 to 7.5U/ml
  • Southern blot: 150mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Other Notes

For life science research only. Not for use in diagnostic procedures.

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pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

wgk_germany

WGK 1

flash_point_f

No data available

flash_point_c

No data available

Osvědčení o analýze (COA)

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