03353583910
Roche
DIG Oligonucleotide Tailing Kit, 2nd generation
sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
41105500
NACRES:
NA.55
Doporučené produkty
usage
sufficient for 25 reactions (100 pmol oligonucleotide per assay; 1 ug of a 30-mer oligonucleotide)
Quality Level
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
greener alternative product characteristics
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
greener alternative category
General description
DIG Oligonucleotide Tailing Kit, 2nd generation employs the enzyme terminal transferase. It catalyzes the addition of digoxigenin (DIG)-11-deoxyuridine triphosphate (dUTP) and deoxyadenosine triphosphate (dATP) to the 3′-OH end of oligonucleotides.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Application
DIG Oligonucleotide Tailing Kit, 2nd generation has been used to label oligonucleotide probes in:
- northern blot assay
- in situ hybridization (ISH)
- fluorescence in situ hybridization (FISH)
Features and Benefits
Tailing of oligonucleotides at the 3′-end with DIG-11-dUTP and recombinant Terminal Transferase. Oligonucleotides are tailed with DIG-dUTP and dATP at an average tail length of 50 nucleotides (tail length range: 10 – 100).
- Very sensitive hybridization probes, due to the incorporation of several DIG-nucleotides
- Fast hybridization kinetics, due to the small size of oligonucleotides
- Single-stranded probes, no renaturation during hybridization
- Sequence can be designed according to the experiment
- Specially suited for in situ hybridization; due to their small size, oligonucleotides readily diffuse into fixed tissues and cells
Packaging
1 kit containing 11 components
Principle
DIG-dUTP and dATP are combined at a concentration that gives the highest DIG incorporation into the tail, and optimal spacing of DIG and dATP, to achieve the highest sensitivity in hybridization experiments. DIG-dUTP and dATP are provided as separate solutions to allow greater flexibility in terms of tail length, hapten spacing, and the use of unlabeled nucleotide(s).
Preparation Note
Working concentration: Oligonucleotides
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.
In one standard labeling reaction up to 100 pmol oligonucleotide (1 μg of a 30-mer oligonucleotide) can be applied.
Storage and Stability
Store at -15–-25 °C. (unopened kit)
Other Notes
For life science research only. Not for use in diagnostic procedures.
Pouze součásti soupravy
Č. produktu
Popis
- Reaction Buffer 5x concentrated
- CoCl<SUB>2</SUB> Solution 25 mM
- DIG-dUTP Solution 1 mM
- dATP Solution 10 mM
- Recombinant Terminal Transferase 400 U/μl
- Control Oligonucleotide, unlabeled 20 pmol/μl
- Oligonucleotide, DIG-dUTP/dATP tailed 2.5 pmol/μl
- Control DNA 0.25 mg/ml
- Glycogen Solution 20 mg/ml
- DNA Dilution Buffer, 50 μg/ml fish sperm DNA
- Poly(A) Solution 10 mg/ml
Zobrazit všechny (11)
signalword
Danger
Hazard Classifications
Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 1B Inhalation - Repr. 1B
Storage Class
6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
Osvědčení o analýze (COA)
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Sortimentní položky
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..
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