MABT118

Anti-Desmoglein-1 Antibody, clone 32-2B

clone 32-2B, from mouse

• Synonyma: Desmoglein-1, Desmosomal glycoprotein 1, DG1, DGI, Pemphigus foliaceus antigen
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Vlastnosti

• biological source: mouse
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 32-2B, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): bovine (immunogen homology)
• technique(s): immunocytochemistry: suitable; immunofluorescence: suitable; western blot: suitable
• isotype: IgG2aκ
• NCBI accession no.: NP_776470
• UniProt accession no.: Q03763
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DSG1(1828)

Popis

General description

Desmoglein-1 (DSG1) is a transmembrane glycoprotein belonging to the family of cadherins. It is one of the main structural components of desmosomal junctions which are abundant in tissues undergoing frequent mechanical stress, such as the heart, epidermis, lymphatic endothelial cells, and meninges. The desmoglein-1 subtype is highly expressed in the epidermis. Like other cadherins, desmoglein-1 contains multiple calcium binding sites and a cell-adhesion recognition site which facilitates intercellular adhesions. Desmoglein-1 is regulated by calcium and PKCα signaling, and is also coupled to intermediate filaments of the cytoskeleton. Desmosomes play a critical role in cell and tissue stability, and may contribute to the progression of cancer.

Immunogen

Bovine Desmoglein-1 from nasal epidermis

Application

Anti-Desmoglein-1, clone 32-2B detects levels of Desmoglein-1 proteins & has been published & validated for use in WB, IF, IHC & ICC.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected Desmoglein-1 in human tonsil tissues.

Western Blot Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in bovine epidermal desmosomal cores and in human keratinocyte cell lysates. (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Immunofluorescent Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in MDCK cells (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Desmoglein-1 in human epidermal tissue (Maruani, A., et al. (2008). Am J Clin Pathol. 130(3):369-374.).

Research Category
Cell Structure

Research Sub Category
ECM Proteins

Quality

Evaluated by Western Blot in human tonsil tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Desmoglein-1 in 10 µg of human tonsil tissue lysate.

Target description

~150 kDa observed. This protein may be glycosolated. Uniprot describes a molecular weight at ~112 kDa; however, Desmoglein-1 has been observed at ~150 kDa (Vilela, M. J., et al. (1995). J Cell Sci. 108(Pt 4):1743-1750.).

Physical form

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human tonsil tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable