MABN328

Anti-peptidyl-citrulline, clone F95 Antibody

clone F95, from mouse

• Synonyma: Peptidyl-citrulline, Protein-bound citrulline
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Clone: F95, monoclonal
• Species reactivity: mouse, rat, chicken, human
• Application: ICC, IHC, WB
• Citations: 53

Vlastnosti

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: F95, monoclonal
• species reactivity: mouse, rat, chicken, human
• species reactivity (predicted by homology): all (based on 100% sequence homology)
• technique(s): immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable
• isotype: IgMκ
• shipped in: wet ice
• target post-translational modification: unmodified

Popis

General description

Deimination (citrullination) is a post translational modification in which the peptidyl-arginine deiminase family of enzymes convert the guanidino side chain of the amino acid arginine into a ureido group, yeilding peptidyl-citrulline. As a result, anti-peptidyl-citrulline antibodies should also cross react with peptidyl-homocitrulline, produced by the carbamylation of lysine, but not free citrulline, which is created in the urea cycle, or as a by-product of nitric oxide synthesis.

Various kDa calculated

Immunogen

Citrullinated peptide consisting of 10 citrulline residues and a carboxyl Gly-Gly-Cys.

Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected peptidyl-citrulline in human cerebellum, and rat cerebellum tissue.
Western Blotting Analysis: A representative lot detected peptidyl-citrulline in CNS proteins from brains and spinal cord of EAE mice (Nicholas et al., 2005).

Research Category
Neuroscience

Research Sub Category
Neurodegenerative Diseases

This Anti-peptidyl-citrulline, clone F95 Antibody is validated for use in Immunohistochemistry and Western Blotting and Immunocytochemistry for the detection of peptidyl-citrulline.

Physical form

Format: Purified

Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry in human cerebral cortex tissue.

Imunnohistochemistry Analysis: A 1:250 dilution of this antibody detected peptidyl-citrulline inhuman cerebral cortex tissue.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Bezpečnostní informace

• Skladovací třída: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable