MAB5268
Anti-Chromogranin A Antibody, clone LK2H10
clone LK2H10, Chemicon®, from mouse
Synonyma:
CgA, Pituitary Secretory Protein I
Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Doporučené produkty
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
clone
LK2H10, monoclonal
species reactivity
porcine, pig, monkey, rat, human, mouse
should not react with
guinea pig, rabbit, sheep
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... CHGA(1113)
General description
Chromogranin A (a protein of 439 amino acids which is encoded on chromosome 14) is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, phenochromocytomas, paragangliomas, and other neuroendocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Specificity
Chromogranin, an acidic protein having a molecular weight of about 68 kDa abides in the polypeptide-hormone-containing secretory granules of human endocrine tissues. Anti-chromogranin A can be used to stain most normal and neoplastic endocrine tissues containing secretory granules (exceptions include cells from the hypothalamus and posterior pituitary, which are known to contain secretory granules). The amount of staining is usually proportional to the number of secretory granules present (1).
Application
Detect Chromogranin A using this Anti-Chromogranin A Antibody, clone LK2H10 validated for use in IH, IH(P) & WB.
Immunohistochemistry:
A 1-10 μg/mL concentration of a previous lot was used in IH.
Optimal working dilutions must be determined by end user.
A 1-10 μg/mL concentration of a previous lot was used in IH.
Optimal working dilutions must be determined by end user.
Packaging
500 μL
Quality
Routinely evaluated by Western Blot on Mouse Pancreas lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected Chromogranin A on 10 μg of Mouse Pancreas lysate.
Western Blot Analysis:
1:1000 dilution of this lot detected Chromogranin A on 10 μg of Mouse Pancreas lysate.
Target description
50 kDa
Linkage
Replaces: CBL219
Physical form
Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.05M Borate buffered saline, 0.15M NaCl, pH 8.2 with 0.1% sodium azide.
Analysis Note
Control
Pancreas, adrenal gland, bowel and thyroid tissues Immunoblot: PC12 cell lysate, human pancreatic tissue
Pancreas, adrenal gland, bowel and thyroid tissues Immunoblot: PC12 cell lysate, human pancreatic tissue
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
recommended
Č. produktu
Popis
Stanovení ceny
signalword
Danger
hcodes
Hazard Classifications
Repr. 1B
Storage Class
6.1D - Non-combustible acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
wgk_germany
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Osvědčení o analýze (COA)
Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.
Již tento produkt vlastníte?
Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.
Fausto J Rodriguez et al.
Brain pathology (Zurich, Switzerland), 19(4), 623-629 (2008-08-20)
Co-deletion of chromosome arms 1p and 19q, characteristic of oligodendroglial tumors, was recently found to be mediated by t(1;19)(q10;p10). To evaluate the prevalence of 1p19q co-deletion and t(1;19) in extraventricular neurocytomas (EVN), we studied tumors from 23 patients, including 13
Specific endocrine tissue marker defined by a monoclonal antibody.
Lloyd, R V and Wilson, B S
Science (New York, N.Y.), 222, 628-630 (1983)
Neuron precursor features of spindle cell oncocytoma of adenohypophysis.
Sanda Alexandrescu,Robert E Brown,Nitin Tandon,Meenakshi B Bhattacharjee
Annals of Clinical and Laboratory Science null
Tieying Hou et al.
Cancer cytopathology, 128(10), 725-732 (2020-06-24)
Insulinoma-associated protein 1 (INSM1) has recently emerged as a reliable nuclear immunostaining marker for detecting neuroendocrine tumors (NETs) in paraffin-embedded surgical samples and cytologic cell blocks, but the reliability of INSM1 staining on cytologic smears is understudied. This study investigated
Mark C Markowski et al.
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