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Merck
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Key Documents

MAB1222A

Sigma-Aldrich

Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase

clone G3, Chemicon®, from mouse

Synonyma:

Anti-TPS1, Anti-TPSB1, Anti-TPSB2, Anti-Tryptase-2

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

conjugate

alkaline phosphatase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

G3, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

isotype

IgG1κ

NCBI accession no.

NM_003294.3

UniProt accession no.

Q15661

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... TPSAB1(7177)

Specificity

Mast cell tryptase. Will show reactivity to basophils but to a lesser degree.

Application

Anti-Tryptase Antibody, Mast Cell, clone G3, Alkaline Phosphatase is an antibody against Tryptase for use in WB, IH.
Immunoblotting & immunohistochemistry at 1:200-1:1,000. For optimal results, tissues or cytospin preparations should be fixed in Carnoy′s Fluid (60% ethanol, 30% chloroform, 10% glacial acetic acid). Not recommended for use on formaldehyde fixed tissue. May be used on thin sections prepared for ultrastructural analysis after fixation of tissue in a modified Karnovsky′s reagent [Lab. Invest. (1988). 58:682-691].

Optimal working dilutions must be determined by end user.
Research Category
Inflammation & Immunology
Research Sub Category
Inflammation & Autoimmune Mechanisms

Physical form

In 0.05M Tris + 0.1M NaCl + 0.001M MgCl2 with 0.1% NaN3, pH=8.0.

Storage and Stability

Maintain at +2-8°C in undiluted aliquots for up to 6 months.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Osvědčení o analýze (COA)

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Premkumar Siddhuraj et al.
Cells, 10(2) (2021-02-07)
The mast cell granule metalloprotease CPA3 is proposed to have important tissue homeostatic functions. However, the basal CPA3 mRNA and protein expression among mast cell populations has remained poorly investigated. Using a novel histology-based methodology that yields quantitative data on
David Lamb et al.
Respiratory research, 22(1), 158-158 (2021-05-24)
RORγt is a transcription factor that enables elaboration of Th17-associated cytokines (including IL-17 and IL-22) and is proposed as a pharmacological target for severe asthma. IL-17 immunohistochemistry was performed in severe asthma bronchial biopsies (specificity confirmed with in situ hybridization).
Premkumar Siddhuraj et al.
Frontiers in immunology, 13, 924244-924244 (2022-08-20)
The mast cell-specific metalloprotease CPA3 has been given important roles in lung tissue homeostasis and disease pathogenesis. However, the dynamics and spatial distribution of mast cell CPA3 expression in lung diseases remain unknown. Using a histology-based approach for quantitative spatial

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