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Merck

IPVH85R

Immobilon®-P Membrane

1 roll, 8.5 cm x 10 m, 0.45 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

Synonyma:

Immobilon® NOW, Immobilon® narrow roll, Western blotting membrane, blotting membrane, transfer membrane

Přihlásit pro zobrazení organizačních a smluvních cen.

Vybrat velikost

1 EA

12 400,00 Kč

12 400,00 Kč


K dispozici k odeslání DNESpodrobné informace



O této položce

UNSPSC Code:
41105339
eCl@ss:
32031613
NACRES:
NB.23
Material:
PVDF membrane, plain filter, white filter
Detection method:
chemiluminescent, colorimetric, fluorometric, radioactive
Pore size:
0.45 μm pore size
Compatibility:
for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

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Název produktu

Immobilon®-P Membrane, PVDF, 0.45 m, 8.5 cm x 10 m roll, 0.45 um ( 0.45 micron filter ) pore size Hydrophobic immobilon-P PVDF Transfer Membrane for western blotting using Chemiluminescence, Chromogenic or Radioactive detection methods. Comes in an 8.5 cm x 10 m roll.

Quality Level

material

PVDF membrane, plain filter, white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable, western blot: suitable

filter L × W

8.5 cm × 10 m

pore size

0.45 μm pore size

capacity

160 μg/cm2 adsorption capacity (insulin), 215 μg/cm2 adsorption capacity (BSA), 294 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

shipped in

ambient

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Tato položka
IPVH07850IPVH304F0IPVH00010
pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

pore size

0.45 μm pore size

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

material

PVDF membrane, white filter, plain filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

material

PVDF membrane, plain filter, white filter

detection method

chemiluminescent, fluorometric, radioactive, colorimetric

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

detection method

chemiluminescent, colorimetric, fluorometric, radioactive

compatibility

for use with Amido black, for use with Colloidal gold, for use with India ink, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Transillumination, for use with CPTS, for use with Ponceau-S red, for use with Coomassie brilliant blue, for use with Toluidine blue

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

compatibility

for use with Amido black, for use with CPTS, for use with Colloidal gold, for use with Coomassie brilliant blue, for use with India ink, for use with Ponceau-S red, for use with Sypro<TMSYMBOL></TMSYMBOL> ruby, for use with Toluidine blue, for use with Transillumination

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

manufacturer/tradename

Immobilon®

General description

Immobilon®-P transfer membrane is a hydrophobic polyvinylidene fluoride (PVDF) microporous membrane with a nominal pore size of 0.45 micron (μm). It is used for the transfer of proteins from a variety of gel matrices. Immobilon®-P transfer membrane is recommended for most western blotting, especially proteins greater than 20 kDa. This membrane displays improved handling characteristics and staining capabilities, a higher signal-to-noise ratio for enhanced sensitivities, and improved solvent resistance, when compared to nitrocellulose membrane.
Primary Binding Mechanisms: Electrostatic, hydrophobic,
Filter Code: IPVH

Application

Immobilon®-P Membrane, PVDF, 0.45 μm, 8.5 cm x 10 m roll has been used in western blot analysis[1][2] and co-immunoprecipitation.[2]

Other Notes

Replaces: INCP00010

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

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Sortimentní položky

A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.

Protokoly

Protokol pro přípravu vzorků pro buněčnou lýzu a účinnou extrakci proteinů z kultivovaných tkání a buněk pro následnou Western blotting.

Na této stránce jsou uvedeny a popsány tři protokoly pro stripování a reprobování membrány Western blotu.

Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

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Související obsah

We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.

Globální číslo obchodní položky

Skladová položkaGTIN
IPVH85R04054839569067

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