Přejít k obsahu
Merck
Všechny fotografie(1)

Hlavní dokumenty

71341

Sigma-Aldrich

pRSFDuet-1 DNA - Novagen

Synonyma:

pRSFDuet-1 Vector

Přihlásitk zobrazení cen stanovených pro organizaci a smluvních cen

Vybrat velikost

10 μG
12 600,00 Kč

12 600,00 Kč


Očekávané datum odeslání25. února 2025podrobné informace



Vybrat velikost

Změnit zobrazení
10 μG
12 600,00 Kč

About This Item

UNSPSC Code:
41106602
NACRES:
NA.51

12 600,00 Kč


Očekávané datum odeslání25. února 2025podrobné informace


form

liquid

Quality Level

manufacturer/tradename

Novagen®

storage condition

OK to freeze

promoter

Promoter name: T7

selection

kanamycin

shipped in

wet ice

Storage temp.

−70°C

General description

pRSFDuet-1 is designed for the co-expression of two target proteins. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (RBS). The vector also carries the pRSF1030 replicon (also known as NTP1), LacI gene, and kanamycin resistance marker.

Application

pRSFDuet-1 DNA - Novagen has been used as a vector:
  • to clone the DNA fragments of defensin α 5 (DEFA5) for recombinant expression[1]
  • to clone the coding region of Mad1597–719[2]
  • to clone the globular domain of Bombyx mori Spc24 (73-162aa) with an N-terminal 6X His-tag to generate the B. mori 6xHis-Spc24(73-162)-Spc25(70-211) pRSF-DUET1 bacterial expression constructs[3]

Warning

Toxicity: Standard Handling (A)

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Osvědčení o analýze (COA)

Vyhledejte osvědčení Osvědčení o analýze (COA) zadáním čísla šarže/dávky těchto produktů. Čísla šarže a dávky lze nalézt na štítku produktu za slovy „Lot“ nebo „Batch“.

Již tento produkt vlastníte?

Dokumenty související s produkty, které jste v minulosti zakoupili, byly za účelem usnadnění shromážděny ve vaší Knihovně dokumentů.

Navštívit knihovnu dokumentů

Qiao Qiao et al.
Experimental cell research, 407(2), 112809-112809 (2021-09-07)
Defensins are highly conserved antimicrobial peptides, which ubiquitously expressed in different species. In addition to the functions in host defense, their aberrant expression have also been documented in cancerous tissue including breast cancer, lung caner and renal carcinoma etc. Whereas
Elyse S Fischer et al.
EMBO reports, 22(7), e52242-e52242 (2021-05-21)
During metaphase, in response to improper kinetochore-microtubule attachments, the spindle assembly checkpoint (SAC) activates the mitotic checkpoint complex (MCC), an inhibitor of the anaphase-promoting complex/cyclosome (APC/C). This process is orchestrated by the kinase Mps1, which initiates the assembly of the
Mohannad Al Chiblak et al.
BMC molecular and cell biology, 20(1), 60-60 (2019-12-21)
ZNF746 and ZNF777 belong to a subset of the large Krüppel-associated box (KRAB) zinc finger (ZNF) transcription factor family. They contain, like four other members in human, an additional conserved domain, the "domain of unknown function 3669" (DUF3669). Previous work
Nuria Cortes-Silva et al.
Current biology : CB, 30(4), 561-572 (2020-02-08)
Accurate chromosome segregation requires assembly of the multiprotein kinetochore complex at centromeres. In most eukaryotes, kinetochore assembly is primed by the histone H3 variant CenH3 (also called CENP-A), which physically interacts with components of the inner kinetochore constitutive centromere-associated network

Questions

  1. Hi, does this plasmid come ready for transformation into cells or does it come lyophilized? Kind regards John

    1 answer
    1. This product is in solution and comes ready for transformation.

      Helpful?

Reviews

No rating value

Active Filters

Náš tým vědeckých pracovníků má zkušenosti ve všech oblastech výzkumu, včetně přírodních věd, materiálových věd, chemické syntézy, chromatografie, analytiky a mnoha dalších..

Obraťte se na technický servis.