650275

Trypsin, Iodination Grade, Human Pancreas

• Synonyma: Trypsin, Iodination Grade, Human Pancreas
• Číslo CAS: 9002-07-7
• Číslo enzymu podle klasifikace EK: 3.4.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00166985
• UNSPSC Code: 12352202
• NACRES: NA.21

Vlastnosti

• assay: ≥95% (SDS-PAGE)
• Quality Level: 200
• form: lyophilized solid
• specific activity: ≥2.5 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated
• solubility: HCl: 2 mM; aqueous buffer, pH 2.5: soluble
• foreign activity: Chymotrypsin, none detected
• shipped in: wet ice
• storage temp.: −20°C

Popis

General description

Native trypsin from human pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues.

Warning

Toxicity: Irritant (B)

Unit Definition

One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of N-benzyl-L-arginine-pNA per min at 25°C, 200 mM Tris-HCl, pH 7.8, 20 mM CaCl₂.

Physical form

Salt-free.

Preparation Note

Prepared from tissue that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following reconstitution, aliquot and freeze (-70°C).

Other Notes

Merck Index13, 9865.
Travis, J., and Roberts, R.C. 1969. Biochemistry 8, 2884.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

when assayed against N-Suc-Ala-Ala-Pro-Phe-pNA (573462)

Bezpečnostní informace

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1