524733

Phosphodiesterase 4D3, His•Tag^®, Human, Recombinant, S. frugiperda

• Synonym(s): PDE4D3, cAMP-specific phosphodiesterase 4D isoform 3
• NACRES: NA.54
• UNSPSC Code: 12352202
• Specific activity: ≥0.25 U/μL
• Recombinant: expressed in insect cells (S. frugiperda)

Properties

• recombinant: expressed in insect cells (S. frugiperda)
• Quality Segment: 100
• form: liquid
• specific activity: ≥0.25 U/μL
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Full-length, recombinant, human phosphodiesterase 4D3 (accession no. L20970) fused at the N-terminus to a His•Tag sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Supplied as a partially purified preparation.

Physical form

In 150 mM NaCl, 10 mM Tris, pH 8.3 .

Preparation Note

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Gibson, L.C.D., et al. 2006. Eur. J. Pharmacol.538, 39.
Xu, R.X., et al. 2000. Science288, 1822.
Nemoz, G., et al. 1996. FEBS Lett.384, 97.

One unit is defined as the amount of enzyme that will convert 1 pmole cAMP to AMP per min at pH 7.5, 30°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable