344906

FURA 2/AM in Solution

• Synonyma: FURA 2/AM in Solution
• Empirický vzorec (Hillův zápis): C₄₄H₄₇N₃O₂₄
• Číslo CAS: 108964-32-5
• Molekulová hmotnost: 1001.85
• MDL number: MFCD00036976
• UNSPSC Code: 12171500
• NACRES: NA.47

Vlastnosti

• assay: ≥95% (HPLC)
• Quality Level: 100
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• fluorescence: λ_ex 335 nm (high); λ_ex 362 nm (low); λ_em 505 nm (high); λ_em 512 nm (low)
• shipped in: ambient
• storage temp.: −20°C
• SMILES string: N(CC(=O)OCOC(=O)C)(CC(=O)OCOC(=O)C)c1c(cc(cc1)C)OCCOc2c3c([o]c(c3)c4ncc([o]4)C(=O)OCOC(=O)C)ccc2N(CC(=O)OCOC(=O)C)CC(=O)OCOC(=O)C
• InChI: 1S/C44H47N3O24/c1-25-7-8-32(46(16-38(53)65-20-60-26(2)48)17-39(54)66-21-61-27(3)49)35(13-25)58-11-12-59-42-31-14-36(43-45-15-37(71-43)44(57)69-24-64-30(6)52)70-34(31)10-9-33(42)47(18-40(55)67-22-62-28(4)50)19-41(56)68-23-63-29(5)51/h7-10,13-15H,11-12,16-24H2,1-6H3
• InChI key: UTBYGIDPBQOHNT-UHFFFAOYSA-N

Popis

General description

Cell-permeable ester form of FURA 2 (Cat. No. 344900). The active dye is rapidly released in the cell by cytoplasmic esterases. Fluorescent calcium chelator (K_d = 224 nM) that offers 30X greater fluorescence intensity that QUIN 2 (Cat. No. 551826). This probe has been used as a probe in many cellular systems, including cardiac myocytes, vascular smooth muscle cells, and medullary collecting tubules. Spectral properties in the presence of low calcium concentrations (excitation max.: 362 nm, emission max.: 512 nm) differ from those during high calcium conditions (excitation max.: 335 nm, emission max.: 505 nm).

Biochem/physiol Actions

Cell permeable: no

Product does not compete with ATP.

Reversible: no

Packaging

Packaged under inert gas

Warning

Toxicity: Irritant (B)

Physical form

A 1 mM solution of FURA 2/AM (Cat. No. 344905) in anhydrous DMSO.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C). Store frozen under an inert atmosphere.

Other Notes

Davis, M.J., et al. 1992. Am. J. Physiol. 263, H1292.
Rao, G.H., et al. 1992. Biochem. Med. Metab. Biol.47, 226.
Schwartz, Y., et al. 1992. Endocrinology131, 772.
Ward, C.A., and Moffat, M.P. 1992. J. Mol. Cell. Cardiol.24, 937.
Janconi, M.E., et al. 1990. J. Cell Biol.110, 1555.
Tsien, R.Y. 1980. Biochemistry19, 2396.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1
• flash_point_f: 188.6 °F - closed cup - (Dimethylsulfoxide)
• flash_point_c: 87 °C - closed cup - (Dimethylsulfoxide)