34115-M

N-Ethylmaleimide

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells.

• Synonyma: N-Ethylmaleimide, NEM
• Empirický vzorec (Hillův zápis): C₆H₇NO₂
• Číslo CAS: 128-53-0
• Molekulová hmotnost: 125.13
• MDL number: MFCD00005509
• UNSPSC Code: 12352111

Vlastnosti

• Quality Level: 100
• assay: ≥98% (HPLC)
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze
• color: white
• solubility: ethanol: 5 mg/mL
• storage temp.: 10-30°C
• InChI: 1S/C6H7NO2/c1-2-7-5(8)3-4-6(7)9/h3-4H,2H2,1H3
• Inchi Key: HDFGOPSGAURCEO-UHFFFAOYSA-N

Popis

General description

Sulfhydryl alkylating reagent that inhibits H⁺-ATPase and suppresses the short circuit current (IC₅₀ = 22 µM) in pancreatic duct cells. Inactivates NADP-dependent isocitrate dehydrogenase. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei. Stimulates arachidonic acid release through activation of PLA₂ in endothelial cells.

Sulfhydryl alkylating reagent. Has been used for blocking sulfhydryl containing reagents which interfere with glucose oxidase assays. Inactivates NADP-dependent isocitrate dehydrogenase. Endonuclease inhibitor. Also a potent inhibitor of both Mg²⁺ and Ca²⁺/Mg²⁺-stimulated DNA fragmentation in rat liver nuclei.

Biochem/physiol Actions

Primary Target
H+-ATPase

Target IC₅₀: 22 µM in suppressing short circuit current

Warning

Toxicity: Corrosive (L)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Huang, Y.C., et al. 1995. Arch. Biochem. Biophys. 316, 485.
Neve, E.P.A., et al. 1995. Biochem. Pharmacol. 49, 57.
Cain, K., et al. 1994. Biochem. Cell Biol.72, 631.
Banger, K.K., et al. 1993. Biochem. J. 290, 199.
Mattingly, J.R., Jr., et al. 1993. J. Biol. Chem. 268, 3925.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• pictograms: GHS06,GHS05
• signalword: Danger
• hcodes: H300,H311,H314,H317
• pcodes: P260 - P270 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Oral - Acute Tox. 3 Dermal - Eye Dam. 1 - Skin Corr. 1B - Skin Sens. 1
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk_germany: WGK 3
• flash_point_f: 163.2 °F - closed cup
• flash_point_c: 72.9 °C - closed cup