220477-M

Chitosanase, Streptomyces sp. N174

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

• Synonyma: Chitosanase, Streptomyces sp. N174, Chitosan N-acetylglucosaminohydrolase
• Číslo CAS: 51570-20-8
• Číslo enzymu podle klasifikace EK: 3.2.1.132 (BRENDA, IUBMB)
• MDL number: MFCD00211797
• UNSPSC Code: 12352202

Vlastnosti

• Quality Level: 100
• form: liquid
• specific activity: ≥25 units/mg protein
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• pI: 7.5 (temperature optimum 65°C for 10 minutes; recommended temperature 37°C)
• foreign activity: chitinolytic ≤0.01%; proteases <0.25 Azocasein U/ml
• storage temp.: −20°C

Popis

General description

Chitosanase, Streptomyces sp. N174, CAS 51570-20-8, is a member of a new class of enzymes with antifungal properties. Efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%).

Native Chitosanase from Streptomyces species. Chromatographically purified. Chitosanase is a member of a new class of enzymes with antifungal properties that efficiently catalyzes the hydrolysis of acetylated chitosan (0 to 60%). Does not hydrolyze chitin or carboxylmethyl cellulose. Acts by splitting the b1,4-glycosidic linkages of GlcN-GlcN (K_m = 29.3 µg/ml) and GlcNAc-GlcN. Its activity generally increases with decreasing acetylation of chitosan. Suitable for the efficient preparation of protoplasts of Mucor and other Zygomycetes, allowing the genetic manipulation of these fungi. Has an optimal pH of 5.5, an optimal temperature of 65°C for 10 min, and a recommended temperature of 37°C.

Warning

Toxicity: Standard Handling (A)

Unit Definition

One Azocasein unit is defined as the amount of protease that will liberate sufficient pigment to increase the optical density at 366 nm by 0.01 after incubation for 30 minutes with Azocasein

One unit is defined as the amount of enzyme that will release 1.0 µmol reducing sugars or glucosamine from chitosan per min at 37°C, pH 5.5.

Physical form

In 50 M sodium acetate, 50% glycerol, pH 5.0.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C).

Other Notes

Boucher, I., et al. 1995. J. Biol. Chem. 270, 31077.
Cuero, R.G., and Osuji, G.O. 1995. Food Addit. Contam. 12, 479.
Fukamizo, T., et al. 1995. Biochem J. 311, 377.
Li, T., et al. 1995. Plant Physiol. Biochem. 33, 599.
Fukamizo, T., et al. 1994. Biochim. Biophys. Acta 1205, 183.
Osswald, W.F., et al. 1994. Plant Cell Physiol. 35, 811.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Bezpečnostní informace

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 1