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Supelco

SeQuant ZIC®-HILIC 200Å (5 µm) HPLC Columns

L × I.D. 5 mm × 0.3 mm guard Columns (5 pc)

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About This Item

UNSPSC Code:
41115709

product name

SeQuant ZIC-HILIC (5µm) 5 x 0.3 mm Guard (5 pc), column L × I.D. 0.5 cm × 0.3 mm

material

stainless steel column

Quality Level

agency

suitable for USP L114

product line

SeQuant

feature

endcapped: no

parameter

400 bar pressure
70 °C max. temp.

technique(s)

HPLC: suitable
LC/MS: suitable (Capillary LC)
mass spectrometry (MS): suitable (High)

column L × I.D.

0.5 cm × 0.3 mm

matrix

fully porous particle

matrix active group

ZIC-HILIC bonding phase

particle size

5 μm

pore size

100 Å pore size

operating pH

2-7.5

separation technique

hydrophilic interaction (HILIC)

storage temp.

no temp limit

General description

The SeQuant ZIC®-HILIC Guard column protects your Capillary column from contamination. The pack contains 5 Guard columns.SeQuant ZIC®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.Thanks to their favorable retention and robust performance, SeQuant HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant ZIC®-HILIC for analysis of melamine and related substances. SeQuant HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

Legal Information

SeQuant is a trademark of Merck KGaA, Darmstadt, Germany
ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany
ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany

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