L040050

Cap A

configured for (ÄKTA^® and OligoPilot^®)

• UNSPSC Code: 12352200
• NACRES: NA.21

Vlastnosti

• type: for DNA synthesis
• Quality Level: 200
• product line: Proligo Reagents
• form: liquid
• storage condition: dry at room temperature
• impurities: ≤100 ppm water content (Karl Fischer)
• color: clear
• suitability: conforms to structure (GC, Formulation)
• compatibility: configured for (ÄKTA^® and OligoPilot^®)
• storage temp.: room temp

Popis

Application

Cap A solution is used after the phosphoramidite reaction/coupling step. This solution aborts chains bearing unreacted 5′hydroxyl groups due to failure of reaction with activated phosphoramidite (typically, 1 to 2% of growing oligonucleotide chains in each synthesis cycle). These unreacted 5′hydroxyl groups are capped with an acetyl group and thus rendered unreactive for subsequent synthesis steps.

Preparation Note

Acetonitrile / N-Methylimidazole 8/2 (v/v)

Other Notes

Cap A solution, containing acetonitrile (ACN) and N-methylimidazole (NMI), is mixed in situ with Cap B1 and Cap B2 solution during the capping (acetylation) reaction. Pyridine is applied as a mild base while NMI provides a powerful acylation catalyst.

Legal Information

OligoPilot is a registered trademark of Cytiva

ÄKTA is a registered trademark of Cytiva

Bezpečnostní informace

• pictograms: GHS02,GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H225,H302 + H332,H311,H314,H361d
• pcodes: P210 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Repr. 2 - Skin Corr. 1B
• Storage Class: 3 - Flammable liquids
• wgk_germany: WGK 2
• flash_point_f: 35.6 °F
• flash_point_c: 2 °C