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T2885

Sigma-Aldrich

3,3′,5,5′-Tetrametilbencidina

TMB substrate, chromogenic, ≥98% (TLC), powder

Sinónimos:

BM azul, Sure Blue TMB, Sustrato TMB, TMB, Transferencia de TMB Plus

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About This Item

Fórmula lineal:
[-C6H2(CH3)2-4-NH2]2
Número de CAS:
54827-17-7
Peso molecular:
240.34
Beilstein/REAXYS Number:
2808541
EC Number:
259-364-6
MDL number:
MFCD00007748
UNSPSC Code:
12352204
PubChem Substance ID:
24900090
NACRES:
NA.83

product name

3,3′,5,5′-Tetrametilbencidina, ≥98% (TLC)

Quality Level

200

assay

≥98% (TLC)

form

powder

mp

168-171 °C (lit.)

solubility

ethyl acetate: 50 mg/mL, clear to very slightly hazy

storage temp.

2-8°C

SMILES string

Cc1cc(cc(C)c1N)-c2cc(C)c(N)c(C)c2

InChI

1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3

Inchi Key

UAIUNKRWKOVEES-UHFFFAOYSA-N

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Application

3,3′,5,5′-Tetramethylbenzidine has been used:
  • as a supplement in Man-Rodosa-Sharpe (MRS) agar plate, to stains the lactobacilli colonies blue in the presence of O2
  • as a substrate solution for ELISA (enzyme-linked immunosorbent assay)
  • in myeloperoxidase activity assay to quantify neutrophil

La 3,3′,5,5′-tetrametilbencidina (TMB) es un sustituto no carcinógeno (prueba de Ames negativa) de la bencina, adecuada como sustrato de la peroxidasa para procedimientos ELISA. Este sustrato rinde un producto final soluble que es de color azul claro y puede leerse espectrofotométricamente a 370 o 620-650 nm. La reacción de la TMB puede detenerse con H2SO4 2 M (que produce un color amarillo) y leerse a 450 nm. Reactivo sensible y específico para la detección de sangre, análisis de la hemoglobina, análisis de peroxidasa.

Biochem/physiol Actions

3,3′,5,5′-Tetramethylbenzidine/TMB can be used as a chromogen to increase the progression of product obtained in peroxidase reaction. In food and environmental decontamination procedures, TMB can be used in in situ free available chlorine (FAC) monitoring of chlorite-based sanitizers.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 4 - Carc. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Ferrotransferrin and antibody against the transferrin receptor as potential vehicles for drug delivery across the mammalian blood-brain barrier into the central nervous system
Methods in Neurosciences, 21(1), 93-117 (1994)
The CD4+ T cell-mediated IFN-gamma response to Helicobacter infection is essential for clearance and determines gastric cancer risk
Sayi A, et al.
Journal of immunology (Baltimore, Md. : 1950), 182(11), 7085-7101 (2009)
Lactobacilli isolated from vaginal vault of dairy and meat cows during progesteronic stage of estrous cycle
Rodriguez C, et al.
Anaerobe, 17(1), 15-18 (2011)
Tetramethylbenzidine method for monitoring the free available chlorine and microbicidal activity of chlorite-based sanitizers under organic-matter-rich environments
Yamaoka H, et al.
Letters in Applied Microbiology, 62(1), 47-54 (2016)
A Felten et al.
Journal of clinical microbiology, 37(3), 729-733 (1999-02-13)
Lactobacilli recovered from the blood, cerebrospinal fluid, respiratory tract, and gut of 20 hospitalized immunocompromised septic patients were analyzed. Biochemical carbohydrate fermentation and total soluble cell protein profiles were used to identify the species. Hydrogen peroxide production was measured. Susceptibility
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