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Merck

P0501

Sigma-Aldrich

Phenolphthalein β-D-glucuronide

β-glucuronidase substrate

Sinónimos:

Phenolphthalein β-D-glucosiduronic acid, Phenolphthalein glucuronic acid, Phenolphthalein mono-β-D-glucosiduronic acid

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About This Item

Fórmula empírica (notación de Hill):
C26H22O10
Número de CAS:
Peso molecular:
494.45
Beilstein/REAXYS Number:
68526
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.32

Quality Level

assay

≥97% (TLC)

form

powder

solubility

H2O: soluble 100 mg/mL

storage temp.

−20°C

SMILES string

O[C@@H]1[C@@H](O)[C@@H](O[C@@H]([C@H]1O)C(O)=O)Oc2ccc(cc2)C3(OC(=O)c4ccccc34)c5ccc(O)cc5

InChI

1S/C26H22O10/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32/h1-12,19-22,25,27-30H,(H,31,32)/t19-,20-,21+,22-,25+,26?/m0/s1

InChI key

FXJYOZKDDSONLX-XADSOVDISA-N

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Application

β-glucuronidase activity was determined by the amount of phenolphthalein released from 0.01 M henolphthalein β-D-glucuronide.
Phenolphthalein β-D-glucuronide has been used as a substrate for the detection of β-D-glucuronidase activity in fecal bacteria.

Biochem/physiol Actions

Phenolphthalein β-D-glucuronide is a chromogenic substrate for β-glucuronidase enzyme and the resultant colored product of the reaction is measured at 552 nm.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


Certificados de análisis (COA)

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Visite la Librería de documentos

A microtitre plate assay for measuring glycosidase activity
Ball Al, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 23(1), 131-135 (2008)
Effects of two pomace insoluble fibres on the activities of faecal bacterial enzymes and intestinal health
Chau CF and Chen CH
European Food Research and Technology, 222(5-6), 681-685 (2006)
Intestinal health benefits of the water-soluble carbohydrate concentrate of wild grape (Vitis thunbergii) in hamsters
Huang YL, et al.
Journal of Agricultural and Food Chemistry, 60(19), 4854-4858 (2012)
Hisashi Satoh et al.
Water science and technology : a journal of the International Association on Water Pollution Research, 83(6), 1399-1406 (2021-03-27)
Monitoring of Escherichia coli concentrations in river water (RW) is essential to identify fecal pollution of the river. The objective of this study was to assess the suitability of a novel, simple and high throughput method developed in our laboratory
Improvement in intestinal function and health by the peel fiber derived from Citrus sinensis L cv Liucheng
Chi-Fai Chau, Fuu Sheu, Ya-Ling Huang and Li-Hung Su
Journal of the Science of Food and Agriculture, 85, 1211-1216 (2005)

Artículos

Drug conjugate analysis and the enzymatic hydrolysis of glucuronides

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Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

Probiotics exhibit an inhibitory effect on pathogens, help prevent chronic intestinal inflammatory diseases or atopic syndromes, and support the immune system.

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Protocolos

Objective: To standardize a procedure for the enzymatic assay of β-Glucuronidase.

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