MTOX1004P24

MDR1/BCRP Double Knockout Caco-2 Cells

human cervix, Epithelial

• Sinónimos: MDR1/BCRP Double Knockout C2BBe1 Cells
• UNSPSC Code: 41106514
• NACRES: NA.81

Propiedades

• product name: MDR1/BCRP Double Knockout Caco-2 Cells, one assay ready, 24 well plate
• biological source: human colon
• form: solid
• morphology: Epthelial
• technique(s): drug transporter assay: suitable; permeability assay: suitable
• application(s): ADME/TOX

Descripción

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1/BCRP double knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the MDR1 and BCRP efflux transporters.

The three week production lead time begins on the Monday following a purchase, in the third week the plates are shipped on Tuesday for receipt on Wednesday or Thursday. As a biologic product that′s shipped at room temperature the cells must be processed immediately upon receipt.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone, C2BBe1 cells, are ideal for transporter analysis as they express multiple transporters, are human derived and grow in a homogenous monolayer that forms tight juntions which is necessary for efflux ratio analysis. Other benefits include:

• Functional knockout of the MDR1 and BCRP genes eliminates the reliance on chemical inhibitors to determine if a compound is an MDR1 or BCRP substrate
• The 24 well Transwell format enables the MDR1/BCRP knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Información de seguridad

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable