The solubility of H375, Hyaluronidase from bovine testes, is 100 mg/mL in water. To achieve a higher solubility, it is advised to warm the tube at 37°C and shake it in an ultrasonic bath for a period. The stock solution can be stored at temperatures below -20°C for several months.
H3757
Hialuronidasa from bovine testes
Type VIII, lyophilized powder, 300-1,000 U/mg
Sinónimos:
Hialuronato 4-glucanohidrolasa, Hialuronoglucosaminidasa
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About This Item
Número de CAS:
Número CE:
Número MDL:
Código UNSPSC:
12352204
NACRES:
NA.54
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origen biológico
bovine testis
Nivel de calidad
conjugado
conjugate (Glucosaminoglycan)
tpo
Type VIII
Formulario
lyophilized powder
actividad específica
300-1,000 U/mg
mol peso
~55 kDa (four subunits of 14 kDa each)
enfermedades relevantes
cancer
temp. de almacenamiento
−20°C
Información sobre el gen
cow ... HYAL1(515397) , HYAL2(281838)
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Acciones bioquímicas o fisiológicas
Estas enzimas rompen aleatoriamente los enlaces glucosídicos β-N-acetilhexosamina [1→4] del ácido hialurónico, la condroitina y los sulfatos de condroitina.
La hialuronidasa degrada el hialuronano y se ha observado que su regulación no es apropiada durante la progresión del cáncer.
Definición de unidad
One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).
Nota de preparación
Prepared from aseptic filled solution of Type I-S.
Otras notas
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
Lot/Batch Number
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Artículos
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Explore the role of hyaluronan, hyaluronic acid structure, hyaluronan synthesis & degradation, and more. Find GAGs, hydrogels, and scaffold kits.
Protocolos
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
To measure hyaluronidase activity, a turbidimetric determination assay is used at 600 nm. One unit of hyaluronidase activity will cause a change in absorbance of 0.330 per minute at pH 5.35 at 37 °C.
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What is the recommended method for reconstituting item H375, Hyaluronidase from bovine testes?
1 answer-
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Good Morning, I our lab we got this product (H3757) and we would like to know how to obtain the package insert since we are not sure how to store it or after open expiration date. Not lot number on the bottle so not sure how to look for it. Thank you
1 answer-
Hyaluronidase from bovine testes may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin. For more information please view the assay below:
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How is product H3757, Hyaluronidase from bovine testes, solubilized?
1 answer-
Product H3757, Hyaluronidase from bovine testes, may be reconstituted at 1 mg/mL in 0.02 M sodium phosphate buffer, pH 7.0 containing 77mM sodium chloride and 0.01% bovine serum albumin.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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