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GE28-4083-47

Ab Spintrap™

Cytiva 28-4083-47

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.25

ligand

recombinant protein G lacking albumin-binding region

envase

pkg of 50 × 100 μL

fabricante / nombre comercial

Cytiva 28-4083-47

condiciones de almacenamiento

(20% Ethanol)

lecho volumen

100 μL

Matriz

highly cross-linked 6% agarose

tam. prom. pieza

34 μm

cleaning

2-9

Intervalo de trabajo

3-9

capacidad

>1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.)
 >1 mg binding capacity(IgG/column)

temp. de almacenamiento

2-8°C

Categorías relacionadas

Descripción general

Ab SpinTrap™ are prepacked, single-use spin columns for rapid purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. Designed for small-scale purification of multiple samples in parallel.
pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

Aplicación

Ab SpinTrap™ is designed for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants. In addition, a protocol for immunoprecipitation is included.


The spin columns are also suitable for use in antibody screening experiments.


Ab SpinTrap™ columns are used together with a standard microcentrifuge and one purification run is performed in less than 20 min.


Ab SpinTrap™ contains Protein G Sepharose™ High Performance, which has high Protein binding capacity and is compatible with all commonly buffers used in antibody purification with immobilized Protein G media.


For optimal performance in antibody purification, use Ab SpinTrap™ together with buffers prepared using Ab Buffer Kit.

Características y beneficios

  • Rapid and convenient small-scale purification of IgG.
  • Protein G Sepharose™ High Performance in prepacked spin columns.
  • No filtration or centrifugation of different crude samples necessary for antibody purification.
  • Classic and crosslink protocols for Protein enrichment (immunoprecipitation).
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.
  • Elution conditions formatted for both LC-MS and electrophoresis analysis.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva
SpinTrap is a trademark of Cytiva

Pictogramas

Flame
GHS02

Palabra de señalización

Warning

Frases de peligro

H226

Código de clase de almacenamiento

3 - Flammable liquids

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Certificados de análisis (COA)

Lot/Batch Number

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Artículos

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

Desalting at laboratory scale is a well-proven, simple, and fast method that will rapidly remove low molecular weight contaminants at the same time as transferring the sample into the desired buffer in a single step.

Protocolos

Performing a Purification of IgG Antibodies with Protein G HP SpinTrap™/Ab SpinTrap™

This page shows how to separate IgG antibodies by affinity chromatography using Protein G HP SpinTrap™/Ab SpinTrap™ from Cytiva.

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