GE28-4013-53

His Spintrap^™

Cytiva 28-4013-53

• Sinónimos: His Spintrap Column
• Código UNSPSC: 41106500
• NACRES: NA.32

Propiedades

• fabricante / nombre comercial: Cytiva 28-4013-53
• envase: pkg of 50 x 100 μL
• lecho volumen: 100 μL
• Matriz: highly cross-linked 6% agarose
• tam. prom. pieza: 34 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• Intervalo de trabajo: 3-12(Ni²⁺-stripped medium.)
• capacidad: ≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descripción

Descripción general

His SpinTrap^™ is a prepacked, single-use spin column for purifying histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC)

* See the section for licensing information in our terms and conditions.

Purification of unclarified samples on His SpinTrap^™ columns minimizes loss of target protein caused by manual operations such as sample precentrifugation, transfer to centrifugation tubes, and collecting supernatant. In addition, loading unclarified sample directly to His SpinTrap^™ columns reduces sample preparation time, which minimizes degradation and oxidation of sensitive target proteins.

His SpinTrap^™ columns are used together with a standard microcentrifuge and one purification run takes approximately 10 min. For optimal performance, use His SpinTrap^™ together with buffers prepared using His Buffer Kit.

The columns are prepacked with Ni Sepharose^™ High Performance, which is precharged with nickel ions for capture of histidine-tagged proteins. Leakage of nickel ions from the Ni Sepharose^™ High Performance medium is negligible.

Características y beneficios

• For simple, small-scale purification of histidine-tagged* proteins and rapid expression screening.
• Ni Sepharose^™ High Performance in prepacked spin columns.
• Yields up to 750 μg pure protein per column.
• Allows direct purification of both clarified and unclarified cell lysates.

Almacenamiento y estabilidad

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (0.15% Kathon CG)

Store at 4 to 30 °C (20% Ethanol)

Nota de análisis

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Información legal

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Información de seguridad

• Pictogramas: GHS09
• Palabra de señalización: Warning
• Frases de peligro: H317
• Consejos de prudencia: P280 - P261 - P272 - P302 + P352 - P333 + P313 - P501
• Código de clase de almacenamiento: 12 - Non Combustible Liquids