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Key Documents

A2382

Sigma-Aldrich

Azoalbumin

protease substrate

Sinónimos:

Sulfanilic acid-azoalbumin

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32

biological source

bovine

Quality Level

form

powder

concentration

60-90% (Lowry)

technique(s)

tissue culture: suitable

solubility

0.01 M HCl: soluble 25 mg/mL

storage temp.

2-8°C

General description

Azoalbumin is a substrate for assaying proteolytic enzymes, majorly trypsin, pepsin and cysteine proteinase. The assay method offers precision and is easy to perform.

Application

Azoalbumin has been used:
  • as a substrate for enzymatic assay of hyphal extracts
  • for screening general protease activity in spent growth medium extract
  • for screening S. rolfsii proteases

Preparation Note

Prepared from Bovine albumin, Fraction V (A 4503)

Substrates

A soluble chromogenic substrate for proteolytic enzymes.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Frontiers in Physiology, 7(3), 53-53 (2016)
Optimisation of cellobiose dehydrogenase production by the fungus Sclerotium (Athelia) rolfsii
Ludwig R and Haltrich D
Applied Microbiology and Biotechnology, 61(1), 32-39 (2003)
A modified azoalbumin technique for the assay of proteolytic enzymes for use in blood group serology.
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