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A2765

Sigma-Aldrich

Azocasein

protease substrate, chromogenic, powder

Sinónimos:

Sulfanilamide-azocasein

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About This Item

Número de CAS:
102110-74-7
MDL number:
MFCD00130592
UNSPSC Code:
12352204
NACRES:
NA.32

product name

Azocasein, protease substrate

biological source

bovine

Quality Level

200

form

powder

technique(s)

ligand binding assay: suitable

solubility

water: 5 mg/mL, clear, orange to very deep orange

ε (extinction coefficient)

≥25 at 440 nm in 0.1 M NaOH at 1%

storage temp.

2-8°C

General description

Azocasein is a chromogenic derivative of casein. Protease degrades azocasein to yield TCA-soluble azopeptides with high UV-absorbance. This azocasein assay is widely employed to estimate the protease production by bacterial fermentation on synthetic substrates from glucose and inorganic salts.

Application

Azocasein has been used as a substrate for determination of protease activity.
Azocasein is a nonspecific protease substrate. Hydrolysis of the casein releases the azo dye into the media where it is detected by absorbance at 440 nm.
Azocasein is an inflammatory agent that is used to induce amyloid A amylooidosis in experimental animals.

Linkage

View more information on azocasein protease assay at www.sigma-aldrich.com/enzymeexplorer

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Nicolas Oswaldo Gomez et al.
Molecular microbiology, 115(1), 84-98 (2020-09-09)
To overcome the metal restriction imposed by the host's nutritional immunity, pathogenic bacteria use high metal affinity molecules called metallophores. Metallophore-mediated metal uptake pathways necessitate complex cycles of synthesis, secretion, and recovery of the metallophore across the bacterial envelope. We
Hongfei Liu et al.
Protein and peptide letters, 27(11), 1102-1113 (2020-03-21)
Protein drugs have disadvantages, such as short half-lives, unstable biological activities, and low utilization efficiency. In this paper, a porous ion-responsive targeted drug delivery system was designed, combining biodegradable carriers with ion exchange technology to overcome problems for protein drug
Azocasein substrate for determination of proteolytic activity: reexamining a traditional method using bromelain samples
Coelho DF, et al.
BioMed Research International, 2016 (2016)
Recombinant proteins from plants: production and isolation of clinically useful compounds, 3 (1998)
Yosuke Tashiro et al.
Journal of bacteriology, 191(24), 7509-7519 (2009-10-20)
The opportunistic human bacterial pathogen Pseudomonas aeruginosa produces membrane vesicles (MVs) in its surrounding environment. Several features of the P. aeruginosa MV production mechanism are still unknown. We previously observed that depletion of Opr86, which has a role in outer
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