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PF0100

Sigma-Aldrich

Protease Fluorescent Detection Kit

high sensitivity assay

Sinónimos:

Protease Assay Kit

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.26

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Application

Fluorescent Protease Detection Kit Video Protocol
Protease Fluorescent Detection Kit has been used:
  • in the detection of proteolytic activity using casein based protease assay
  • for testing the extracellular proteolytic activity of fungal strains
  • for quantifying mitochondrial protease activity
  • for measuring the protease production from soil solution

Suitability

Detection limit: approx. 50 ng trypsin

Quantity

Supplied with sufficient reagents for up to 200 one mL assays.
Supplied with sufficient reagents for up to 200 one mL assays.

The trichloroacetic acid (TCA) component is provided as 6.1 N TCA solution. 0.6 N TCA Solutions may be prepared by a 10-fold dilution of the desired volume of T0699 with ultrapure water. It is not necessary to dilute the entire volume of T0699 for immediate use of the kit.

Analysis Note

Tested for detection of all four proteolytic classes (serine, apartic, cysteine and metalloproteinases).

Solo componentes del kit

Referencia del producto
Descripción
  • Incubation buffer 25 mL
  • FITC-casein solution 5 mL
  • Assay buffer 200 mL
  • Protease standard (trypsin) 20 μg
  • FITC control 50 mg
  • Trichloroacetic acid (TCA) solution 30 mL

signalword

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1A - Skin Sens. 1 - STOT SE 3

target_organs

Respiratory system

Storage Class

8A - Combustible corrosive hazardous materials

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Caveolin-1 controls mitochondrial function through regulation of m-AAA mitochondrial protease
Volonte D, et al.
Aging (Albany. NY.), 8(10), 2355-2355 (2016)
Selena Gimenez-Ibanez et al.
PLoS biology, 12(2), e1001792-e1001792 (2014-02-22)
Pathogenicity of Pseudomonas syringae is dependent on a type III secretion system, which secretes a suite of virulence effector proteins into the host cytoplasm, and the production of a number of toxins such as coronatine (COR), which is a mimic
Martin H Kunzmann et al.
Molecular bioSystems, 8(11), 3061-3067 (2012-09-20)
α-Alkylidene-γ-butyrolactones are quite common in nature and exhibit a broad spectrum of biological activities. We therefore synthesized a small library of xanthatine inspired α-alkylidene-γ-butyrolactones to screen non-pathogenic and uropathogenic E. coli strains by activity based protein profiling (ABPP). The identified
Joseph S Rom et al.
Virulence, 8(8), 1776-1790 (2017-09-15)
Staphylococcus aureus causes acute and chronic forms of infection, the latter often associated with formation of a biofilm. It has previously been demonstrated that mutation of atl, codY, rot, sarA, and sigB limits biofilm formation in the USA300 strain LAC
Contrasting effects of increased carbon input on boreal SOM decomposition with and without presence of living root system of Pinus sylvestris L.
Linden, A., et al.
Plant and Soil, 377, 145-158 (2014)
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Protocolos

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Protease Fluorescent Detection Kit Usage

Protease Fluorescent Detection Kit detects protease activity using FITC-labeled casein substrate.

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

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