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Merck

57620

Sigma-Aldrich

Inulinase from Aspergillus niger

lyophilized, powder, brown-gray, ~25 U/mg

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About This Item

Número de CAS:
Comisión internacional de enzimas:
EC Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Aspergillus niger

Quality Level

form

powder

quality

lyophilized

specific activity

~25 U/mg

color

brown-gray

storage temp.

2-8°C

General description

Inulinases are classified as exoinulinases and endoinulinases.

Application

Inulinase from Aspergillus niger has been used as a glycoside hydrolase (GH) to study its potential to disperse bacteria (P. aeruginosa and S. aureus) from microbial biofilms using a polymicrobial well-plate model.

Biochem/physiol Actions

Inulinase is used in high-fructose syrup, which has therapeutic applications. Inulinase is reported to have high activity toward sucrose.
Inulinase hydrolyses inulin to produce oligosaccharides and liberate fructose. It also splits the terminal fructose units into sucrose and raffinose.

Unit Definition

1 U corresponds to the amount of enzyme which releases 1 μmol of reducing sugar (measured as fructose) per minute at pH 4.1 and 37°C from inulin

Other Notes

Characterization

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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R. Azhari et al.
Biotechnology and Applied Biochemistry, 11, 105-105 (1989)
Aline Richetti et al.
Bioprocess and biosystems engineering, 35(3), 383-388 (2011-08-13)
An experimental design was carried out to evaluate the effect of the concentrations of sodium alginate, glutaraldehyde and activated coal on the immobilization of inulinase from Kluyveromyces marxianus NRRL Y-7571. The experimental condition of 20 g/L of sodium alginate, 50 mL/L of
L Wang et al.
Journal of applied microbiology, 111(6), 1371-1380 (2011-09-08)
The aim of this study is to improve exoinulinase production by expression of a cloned exoinulinase gene inuA1 (GenBank accession no. JF961344) from Penicillium janthinellum strain B01 in Pichia pastoris. A full-length cDNA of exoinulinase gene (inuA1) was cloned from
Nannan Li et al.
Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 27(7), 1032-1039 (2011-10-25)
Ethanol fermentation from Jerusalem artichoke tubers by recombinant Saccharomyces cerevisiae strains expressing the inulinase gene (inu) from Kluyveromyces marxianus was investigated. The inu native and pgk promoters were used to drive the expression of the inu gene, and the inulinase
C Neagu Bonciu et al.
Letters in applied microbiology, 55(3), 195-201 (2012-06-26)
A newly isolated strain of Rhizoctonia ssp. was used for the production of extracellular inulinase. Previously, the qualitative effects of some carbon and nitrogen sources from fermentative media and the physicochemical parameters for growth were established by Plackett-Burman analysis, and

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