11426338910
Roche
Anti-Fluorescein-AP, Fab fragments
from sheep
Sinónimos:
antibody
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About This Item
Código UNSPSC:
12352203
Productos recomendados
origen biológico
sheep
Nivel de calidad
conjugado
alkaline phosphatase conjugate
forma del anticuerpo
affinity purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
solution
envase
pkg of 150 U (200 μl)
fabricante / nombre comercial
Roche
isotipo
IgG
temp. de almacenamiento
2-8°C
Descripción general
Fluorescein is synthesized from m-dialkylaminophenols or resorcinols condensed with phthalic anhydrides under harsh conditions. Fab fragments bind to G proteins.
This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional activator after forming a protein complex with other proteins. This protein acts as a nucleocytoplasmic shuttle protein and is important for neural crest and peripheral nervous system development. Mutations in this gene are associated with Waardenburg-Shah and Waardenburg-Hirschsprung disease. (provided by Ref Seq)
Especificidad
The polyclonal antibody reacts with free and bound fluorescein.
Aplicación
Use Anti-Fluorescein-AP, Fab fragments for the detection of fluorescein-labeled compounds using:
- Dot blot
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Southern blot
- Western blot
- Electrochemical biosensing technique for the detection of 16S rRNA
Acciones bioquímicas o fisiológicas
Anti-Fluorescein-AP, Fab fragments has been used in oligonucleotide capture, denaturation and detection (CDD).
Características y beneficios
Contents
Solution, stabilized
Solution, stabilized
Nota de preparación
- Working concentration: The following concentrations depend on application and substrate and should be taken as a guideline. Dot blot: 150mU/ml
- ELISA: 150 to 300mU/ml
- Immunohistocytochemistry: 250 to 500mU/ml
- In situ hybridization: 1.5 to 7.5U/ml
- Southern blot: 150mU/ml
- Western blot: 250 to 500 mU/ml
Working solution: 100mM Tris-HCl, 150mM NaCl, pH 7.5. If necessary 1% Blocking reagent (w/v), dry milk powder, 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.
Otras notas
For life science research only. Not for use in diagnostic procedures.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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