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Key Documents

566460

Sigma-Aldrich

Normal Donkey Serum, Sterile

Sinónimos:

Normal Serum

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.47

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

shipped in

wet ice

storage temp.

−20°C

General description

Normal carrier sera, produced from pooled lots of sera from healthy, non-immunized donkeys.
Normal donkey serum is a common blocking agent used to reduce background staining by preventing non-specific binding of antibodies to tissue or fragment crystallizable (FC) receptors.

Application

Normal Donkey Serum, Sterile has been used to reduce artificial staining of non-specific proteins during double-immunofluorescent labeling. It is also suitable for use in radioimmunoassay.

Warning

Toxicity: Standard Handling (A)

Physical form

pH 7.0-8.5.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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R F Holcombe et al.
Molecular pathology : MP, 55(4), 220-226 (2002-07-31)
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Igor Buchwalow et al.
Scientific reports, 1, 28-28 (2012-02-23)
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports. Background staining is thought to occur as a result of either non-specific antibody (Ab) binding to endogenous Fc receptors (FcRs) or a combination of ionic and
Woo Jung Cho et al.
Journal of cellular and molecular medicine, 11(5), 1069-1086 (2007-11-06)
Matrix metalloproteinase-2 (MMP-2) may play roles at intracellular and extracellular sites of the heart in ischaemia/reperfusion injury. Caveolins (Cav-1, -2 and -3) are lipid raft proteins which play roles in cell sig-nalling. This study examined, using immunohistochemistry and two photon

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