The albumin is heat shock fractionated from bovine serum. No buffer is added during this process.
A9647
Seroalbúmina bovina
heat shock fraction, pH 7, ≥98%
Sinónimos:
Albúmina bovina, Albúmina sérica bovina, BSA
About This Item
Productos recomendados
origen biológico
bovine
Nivel de calidad
Ensayo
≥98%
Formulario
lyophilized powder
mol peso
~66 kDa
purificado por
heat shock fractionation
origen
USA origin
técnicas
immunocytochemistry: suitable
impurezas
≤5.0% Loss on drying
pérdida
≤5%
pH
7
solubilidad
water: soluble (40 mg/ml)
Nº de acceso UniProt
actividad extraña
BT virus, none detected
VSV virus, none detected
temp. de almacenamiento
2-8°C
Información sobre el gen
bovine ... ALB(280717)
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Descripción general
Aplicación
- La BSA se ha utilizado en cultivos celulares, por ejemplo, como suplemento en medios preparados para el cultivo de oligodendrocitos.[4]
- La BSA se ha utilizado para la dilución de las disoluciones de anticuerpo primario en los procedimientos inmunohistoquímicos.[5]
- La BSA se ha utilizado como patrón en el método Bradford de cuantificación de proteínas.[6]
- La BSA se ha utilizado en la disolución amortiguadora de bloqueo y permeabilización durante los estudios de inmunofluorescencia.[7]
Acciones bioquímicas o fisiológicas
Nota de preparación
también adquirido normalmente con este producto
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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Certificados de análisis (COA)
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Artículos
This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.
Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.
Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.
Experience effective immobilization of protein A on wide-pore epoxy-modified silica monoliths for mAb separation, showcasing the efficiency of Chromolith® WP 300 columns in achieving faster and highly reproducible biomolecule separations.
Protocolos
To measure alcohol dehydrogenase activity, this assay uses β-nicotinamide adenine dinucleotide phosphate and a continuous spectrophotometric rate determination at 340 nm.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.
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What buffer the buffer it is lyophilized in?
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Is this product protease-free?
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This product has not been tested for protease activity. Please see the link below to review other BSA products that are listed as protease-free:
https://www.sigmaaldrich.com/product/compare?term=&focus=products&page=1&perpage=30&sort=relevance&type=product&products=A7030%3ASIGMA%3Asial%2CA3059%3ASIGMA%3Asial%2CA3294%3ASIGMA%3AsialHelpful?
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Is this product suitable for using as protein standard when measuring concentration by spectrophotometry?
1 answer-
This product has been cited for as use protein standard in Bradford's method for spectrophotometric determination of protein. The article is accessible at this link: https://www.sciencedirect.com/science/article/pii/S0946672X20302698
Please refer to the table for a description of each albumin product. Based on customer input and literature reports, the following table lists product numbers which have successfully been used for specific applications. The list is not comprehensive, and product numbers not listed may often be substituted. It is available at this link: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/111/538/albumin-application-selection-guide.pdf
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What is the difference between Albumin A7906 and Albumin A9647 ?
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Product A9647 and A7906 are identical. The only difference is that product A7906 offers 5 KG and 10 KG pack sizes.
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What is the molecular weight of Product A9647, Albumin from bovine serum?
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The molecular weight of BSA is 66,430.
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How can the concentration of a solution of Product A9647, Albumin from bovine serum, be quantified?
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The concentration of a solution of BSA can be measured by using the extinction coefficient (E1%) of 6.67. The 1% refers to a solution that is 1% weight-to-volume (i.e., 10 mg/mL).
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What is the pH of a solution of Product A9647, Albumin from bovine serum?
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The pH of a 1% solution is approximately 7.0, according to the product specifications.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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