SRP6415

Cathepsin D human

recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

• Sinónimos: CLN10, CPSD, CTSD
• Código UNSPSC: 12352200
• NACRES: NA.32

Propiedades

• origen biológico: human
• recombinante: expressed in HEK 293 cells
• etiqueta: 6-His tagged (C-terminus)
• Ensayo: ≥95% (SDS-PAGE)
• Formulario: lyophilized
• mol peso: calculated mol wt 43.6 kDa; observed mol wt 45-55 kDa (DTT-reduced. Protein migrates due to glycosylation. Ser 19 is the predicted N-terminal.)
• envase: pkg of 10 μg
• fabricante / nombre comercial: Sigma-Aldrich
• condiciones de almacenamiento: dry at room temperature
• técnicas: activity assay: suitable
• impurezas: <1 EU/μg endotoxin (LAL test)
• idoneidad: suitable for molecular biology
• Nº de acceso UniProt: P07339
• aplicaciones: life science and biopharma
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• Información sobre el gen: human ... CTSD(1509)

Descripción

Descripción general

Research area: Cell signalling. Cathepsin D belongs to the peptidase A1 family, an estrogenic-induced lysosomal protease. Cathepsin D can be cleaved into the following 2 chains: N-terminal light chain and C-terminal heavy chain, which is expressed in the aorta extracellular space (at the protein level). ). It is found in most mammalian cells and is located in thelysosomes.

This gene is mapped to human chromosome 11p15.5.

Aplicación

Cathepsin D has been used in the protease digestion of haemoglobin.

Acciones bioquímicas o fisiológicas

Cathepsin D (CatD) takes part in the intracellular degradation of advanced glycation end (AGE) products. AGE deposition is predominant in photoaged skin. Thus, CatD might be useful in antiphotoaging therapy. Elevated circulating CatD is observed in type 2 diabetes and can be considered an important biomarker for type 2 diabetes-dependent cardiac dysfunction. Increased CatD, is observed in non-alcoholic steatohepatitis.Cathepsin D is involved in various physiological processes like apoptosis, autophagy, and protein degradation. Cathepsin D plays an important role in the pathogenesis of Alzheimer’s disease, neuronal ceroid lipofuscinosis, and breast cancer.

Forma física

Lyophilized from 0.22 μm filtered solution in 50 mM MES, pH 6.5 with 100 mM NaCl. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.

Reconstitución

Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable