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Merck

U5382

Sigma-Aldrich

Ubiquitin human

≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), lyophilized powder

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About This Item

Número de CAS:
MDL number:
UNSPSC Code:
41106500
NACRES:
NA.32

biological source

human

Quality Level

recombinant

expressed in E. coli (N-terminal FLAG® tagged)

assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

10 kDa

technique(s)

mass spectrometry (MS): suitable

solubility

0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless
0.05 M Tris pH 7.5: ≥10 mg/mL

UniProt accession no.

storage temp.

−20°C

Gene Information

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General description

Research Area: CANCER
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.
Ubiquitin is a highly conserved globular protein and has lysine in the surface. The C-terminal end comprises the Leu-Arg-Gly-Gly structural motif.

Application

Ubiquitin human can be used as a standard for mass spectrometry.
Ubiquitin human has been used in mono-ubiquitination of yeast proliferating cell nuclear antigen (PCNA) and in in vitro ubiquitination assay of defective in mitotic arrest 1 (Dma1)
Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies.

Biochem/physiol Actions

Ubiquitin interacts with the lysine residue of proteins through its ε-amino group of the C-terminal glycine residue. Proteins interacting with ubiquitin either undergo mono-ubiquitination or multi-mono-ubiquitination via a three-step process. Ubiquitination regulates intracellular trafficking and protein degradation and an imbalance in the pathway is implicated in disorders.
Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.
Ubiquitin-mediated proteolysis plays an important role in several basic cellular processes including regulation of cell cycle and division, differentiation and development, modulation of cell-surface receptors, the secretory pathway (protein transport), morphogenesis of neuronal networks, transcriptional regulation and signal transduction, transcriptional silencing, DNA repair, long-term memory, and circadian rhythms.

Packaging

Package size based on protein content

Preparation Note

Ubiquitin human can be dissolved in 0.05 M Tris-HCl at a concentration of 10.00 - 11.00 mg/ml to yield a clear to slightly hazy, colorless solution.

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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The ubiquitin-proteasome pathway and the regulation of growth hormone receptor availability
Strous, Ger J and van Kerkhof, Peter
Molecular and cellular endocrinology, 143-151 (2002)
Ana Camara-Artigas et al.
Acta crystallographica. Section F, Structural biology communications, 72(Pt 1), 29-35 (2016-01-12)
Ubiquitin is a small globular protein that has a considerable number of lysine residues on its surface. This results in a high surface entropy that precludes the formation of crystal-packing interactions. To date, only a few structures of the native
The ubiquitin-proteasome pathway and the regulation of growth hormone receptor availability
Strous, Ger J and van Kerkhof, Peter
Molecular and Cellular Endocrinology, 143-151 (2002)
Regulation of NF-kappaB by ubiquitination
Chen J and Chen ZJ
Current Opinion in Immunology, 4-12 (2013)
David Komander
Biochemical Society transactions, 37(Pt 5), 937-953 (2009-09-17)
Protein ubiquitination and protein phosphorylation are two fundamental regulatory post-translational modifications controlling intracellular signalling events. However, the ubiquitin system is vastly more complex compared with phosphorylation. This is due to the ability of ubiquitin to form polymers, i.e. ubiquitin chains

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