MWGF1000
Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da
Sinónimos:
Gel Filtration Markers Kit, Gel Filtration Protein Ladder, Protein Molecular Weight Marker Kit
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About This Item
Código UNSPSC:
12352200
NACRES:
NA.25
En este momento no podemos mostrarle ni los precios ni la disponibilidad
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Formulario
liquid
Nivel de calidad
técnicas
liquid chromatography (LC): suitable
temp. de almacenamiento
−20°C
Descripción general
Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.[1]
Aplicación
Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da has been used:
- for protein purification and analyses (gel-filtration experiments)[2]
- for size-exclusion chromatography/gel filtration chromatography[3][4] of synaptoneurosome extract[5]
- for glycerol gradient centrifugation[6]
- to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system
- to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner
Acciones bioquímicas o fisiológicas
The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.
Otras notas
- For research use only. Not for drug, household, or other uses.
- Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
- Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- A8531Albumin, bovine serum, 66,000 MW 1 mL/vialSDS
- A8656Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vialSDS
- A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vialSDS
- A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vialSDS
- D4772Blue dextran, 2,000,000 MW 1 mL/vialSDS
- C7025Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vialSDS
- T9145Thyroglobulin, bovine, 669,000 MW 1 mL/vialSDS
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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HSP100 proteins are molecular chaperones involved in protein quality control. They assist in protein (un)folding, prevent aggregation, and are thought to participate in precursor translocation across membranes. Caseinolytic proteins ClpC and ClpD from plant chloroplasts belong to the HSP100 family.
D K Wimsatt et al.
Clinical chemistry, 33(11), 2100-2106 (1987-11-01)
The Coomassie Brilliant Blue G-250 method for urinary proteins underestimates urinary immunoglobulin light chains when albumin or pooled serum is used as the protein standard. The specific color yields of these and other proteins can be brought closer together by
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 24(12), 5013-5023 (2010-08-25)
Voltage-gated calcium channels conduct Ca(2+) ions in response to membrane depolarization. The resulting transient increase in cytoplasmic free calcium concentration is a critical trigger for the initiation of such vital responses as muscle contraction and transcription. L-type Ca(v)1.2 calcium channels
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Nucleic acids research, 48(17), 9589-9605 (2020-08-29)
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Gel-filtration chromatography
F'ag'ain 'O?C, et al.
Journal of biological and chemical chronicles, 25-33 (2011)
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