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S5897

Sigma-Aldrich

Sephadex® G-50

BioReagent, for molecular biology, DNA grade, fine

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About This Item

Número de CAS:
9004-54-0
MDL number:
MFCD00132771
UNSPSC Code:
23151817
NACRES:
NA.52

grade

DNA grade
for molecular biology

product line

BioReagent

form

beads

technique(s)

DNA purification: suitable

matrix

cross-linked dextran

swelling

1 g swells to 9-11 mL

bead diameter

20-80 μm

pore size

exclusion limit (20 bp dsDNA (rA)20)

pH

2-10

foreign activity

DNase, RNase, none detected

storage temp.

room temp

InChI

1S/C18H32O16/c19-1-5(21)9(23)10(24)6(22)3-31-17-16(30)14(28)12(26)8(34-17)4-32-18-15(29)13(27)11(25)7(2-20)33-18/h1,5-18,20-30H,2-4H2

InChI key

FZWBNHMXJMCXLU-UHFFFAOYSA-N

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General description

Sephadex® G-50, Fine is an often-cited gel filtration medium for desalting and buffer exchange of biomolecules > 30,000 MW. Sephadex® is prepared by crosslinking dextran with epichlorohydrin. Sephadex products differ in their degree of cross-linking and hence in their degree of swelling and their molecular fractionation range. “Fine” indicates a small particle size known for excellent efficiency and short diffusion distances.

S5897-100G′s updated product number is GE17-0573-02

Application

Sephadex® G-50 is suitable for spin column purification of DNA (from contaminating small molecules) for use in hybridizations. It was used to purify PCR products prior to DNA sequencing to improve the sequencing quality.

Features and Benefits

  • Excellent recovery
  • Minimal sample dilution
  • Quick and easy to use

Legal Information

Sephadex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Stutter products are a common artifact in the PCR amplification of frequently used genetic markers that contain mononucleotide simple sequence repeats. Despite the importance of accurate determination of nucleotide sequence and allele size, there has been little progress toward decreasing
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Applied and environmental microbiology, 75(22), 7189-7195 (2009-09-22)
Streams are highly heterogeneous ecosystems, in terms of both geomorphology and hydrodynamics. While flow is recognized to shape the physical architecture of benthic biofilms, we do not yet understand what drives community assembly and biodiversity of benthic biofilms in the
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The LAGLIDADG homing endonuclease (LHE) I-AniI has adopted an extremely efficient secondary RNA splicing activity that is beneficial to its host, balanced against inefficient DNA cleavage. A selection experiment identified point mutations in the enzyme that act synergistically to improve
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