The control primers specific for the mouse GAPDH promoter are as follows:
FOR: 5'-CCTCTGCGCCCTTGAGCTAGGA-3’
REV: 5'-CACAAGAAGATGCGGCCGTCTC-3’
RIP
Imprint® RNA Immunoprecipitation Kit
High-capacity Protein A magnetic beads for successful RNA Immunoprecipitation,suitable for use with mRNA and microRNA
Sinónimos:
Magnetic Bead RNA Immunoprecipitation, RNA Immunopreciptation, mRNA Immunopreciptation, microRNA Immunoprecipitation
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About This Item
Código UNSPSC:
41116158
NACRES:
NA.54
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Descripción general
Our Imprint RNA Immunoprecipitation Kit provides all reagents needed for successful RIP when paired with an appropriate antibody. The kit includes two lysis buffers, one mild for less tightly bound RNPs, the other harsh for strongly associated RNPs. Protein A magnetic beads are provided to collect immune precipitates and facilitate washing. The kit also includes both rabbit and mouse IgG for negative control RIPs, a rabbit anti-mouse bridging antibody to bind mouse monoclonal primary antibodies efficiently to protein A on the magnetic beads, as well as both protease and ribonuclease inhibitors to protect the RNPs from degradation during RIP.
Learn more about this product and view application data.
Learn more about this product and view application data.
RNA immunoprecipitation (RIP) is a very powerful procedure for the study of RNA binding proteins (RBPs) and their RNA targets in ribonucleoprotein (RNP) complexes. Antibodies raised against specific RBPs are used to coprecipitate RNPs, i.e., the RBP along with its RNA partner. The RNA can then be identified by next generation sequencing, or if testing for a specific RNA, by RT-PCR.
Aplicación
Imprint® RNA Immunoprecipitation Kit has been used for:
- Suitable for downstream applications
- Individual target characterization to genome-wide profiling techniques[1]
- Characterization of signal transduction pathways
- Verification of ChIp-chIP and ChIP-seq data
Características y beneficios
- Xtra Protein A magnetic beads with higher binding capacity
- Step-by-step protocols for cell lysis and immunoprecipitation
- RNAse inhibitors and RNAse-free reagents to maintain RNA integrity
- Negative control and antibody included
Información legal
Imprint is a registered trademark of Merck KGaA, Darmstadt, Germany
Los componentes del kit también están disponibles por separado
Referencia del producto
Descripción
SDS
- I5381IgG from mouse serum, reagent grade, ≥95% (SDS-PAGE), lyophilized powderSDS
- I5006IgG from rabbit serum, reagent grade, ≥95% (SDS-PAGE), essentially salt-free, lyophilized powderSDS
- M7023Anti-Mouse IgG (whole molecule) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solutionSDS
- P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solutionSDS
- R1158Ribonuclease Inhibitor, recombinant, expressed in E. coli, 20-40 units/μLSDS
Opcional
Referencia del producto
Descripción
Precios
obligatorio pero no suministrado
Referencia del producto
Descripción
Precios
Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Aquatic Chronic 3 - Eye Irrit. 2 - Flam. Liq. 3 - Skin Irrit. 2
Código de clase de almacenamiento
3 - Flammable liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
100.4 °F
Punto de inflamabilidad (°C)
38 °C
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Artículos
Sigma’s Imprint RNA Immunoprecipitation Kit was used to copurify human argonaute 2 (Ago2)-associated RNAs from HeLa cells. MicroRNAs reverse transcribed and quantitating using Mysticq reagents.
Protocolos
Procedure and protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit
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Please suggest the appropriate control primer set recommended for use with mouse tissue samples while using EZ Magna Chip A/G kit, 17-10086. It includes control antibodies (anti-RNA pol II) and a control primer set intended for the human GAPDH promoter.
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