L4895

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

• Sinónimos: WGA, Wheat germ agglutinin
• UNSPSC Code: 12352202
• NACRES: NA.32

Propiedades

• conjugate: FITC conjugate
• Quality Level: 200
• form: lyophilized powder
• potency: <50 μg/mL agglutination activity
• composition: Protein, ~50% Lowry
• extent of labeling: ~2 mol FITC per mol protein
• storage temp.: 2-8°C

Descripción

General description

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Application

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine

Lectin from Triticum vulgaris (wheat) has been used:

• to treat transfected cells for immunofluorescence
• to stereotaxically inject into mice for neuronal tracing
• in immunohistochemistry for the analysis of myocytes

Biochem/physiol Actions

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Packaging

Package size based on protein content.

Physical form

Contains phosphate buffer salts

Preparation Note

Conjugates are prepared from affinity purified lectin.

Información de seguridad

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)