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Key Documents

GE17-1166-01

Sigma-Aldrich

HiPrep 16/60 Sephacryl® S-200 HR

Cytiva 17-1166-01, column L × I.D. 60 cm × 16 mm, 50 μm avg. part. size

Sinónimos:

Sephacryl S-200 column

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

polypropylene

mol wt

5000-250000 Da (globular proteins)

packaging

pkg of 1 ea

manufacturer/tradename

Cytiva 17-1166-01

parameter

<1 mL/min flow rate
<5 mL sample volume
0.15 Mpa (22 psi)
22 psi

bed volume

120 mL

column L × I.D.

60 cm × 16 mm

matrix

dextran-acrylamide particle platform
allyl dextran and N, N’-methylenebisacrylamide
polymeric

matrix active group

dextran-acrylamide phase

avg. part. size

50 μm

cleaning

2-13

working range

3-11

separation technique

size exclusion (SEC)

General description

HiPrep 16/60 Sephacryl® S-200 HR are prepacked gel filtration column for preparative separation of biomolecules from up to 5 mL sample volume. Sephacryl® S-200 is used for separating midsize proteins including antibodies and serum proteins.

Features and Benefits

  • Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/bisacrylamide matrix.
  • Gel filtration columns for low to medium pressure AKTA systems.
  • Well-established in industrial production.
  • The HiPrep Sephacryl® series offers reproducible separations over a wide molecular weight range.
  • Convenient, prepacked HiPrep Sephacryl® columns speed up protein purification.

Suitability

All commonly used buffers, 0.2 M NaOH, 0.1 M HCl, 1 M acetic acid, 8 M urea, 6 M guanidine HCl, 1 % SDS, 20 % ethanol, 30 % propanol, 30 % acetonitrile (tested at 40oC for 1 week), 0.5 M NaOH (only for cleaning-in-place)
Gel filtration columns for low to medium pressure ÄKTA systems.
for preparative separation of biomolecules of up to 5 mL sample volume

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

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Legal Information

HiPrep is a trademark of Cytiva
Sephacryl is a registered trademark of Cytiva

pictograms

Flame

signalword

Warning

hcodes

Storage Class

3 - Flammable liquids


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Protocolos

This page shows how to refold proteins using gel filtration and products from Cytiva.

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Sephacryl column separates using Size Exclusion Chromatography media, prepared by covalently cross-linking allyl dextran.

Ver todo

Contenido relacionado

Separation options for Size Exclusion Chromatography with Sephacryl from Cytiva.

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