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A50120

Sigma-Aldrich

DEAE–Sephadex®

Sinónimos:

Diethylaminoethyl Sephadex®

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About This Item

Número de CAS:
39455-31-7
Número MDL:
MFCD00132778
Código UNSPSC:
47101511
NACRES:
NA.56
En este momento no podemos mostrarle ni los precios ni la disponibilidad

Formulario

powder

Nivel de calidad

200

técnicas

protein purification: suitable

Matriz

cross-linked dextran

tamaño microesfera

40-125 μm (dry)

tamaño de poro

~200,000 Da exclusion limit

pH

2—12

capacidad

3-4 meq/g binding capacity

Categorías relacionadas

Descripción general

A50120-100G′s updated product number is GE17-0180-01
A50120-500G′s updated product number is GE17-0180-02

Aplicación

DEAE Sephadex can be used in ion exchange chromatography for purifying and isolating proteins. In has been used to identify and label an insulin activated nitric oxide synthase inhibitor (protein) in acute myocardial infarction (AMI) patients.

Información legal

Sephadex is a registered trademark of Cytiva

sustituido por

Referencia del producto
Descripción
Precios

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, type N95 (US)

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Certificados de análisis (COA)

Lot/Batch Number
0000307352
0000324823
0000267114
SLCP6313
SLCL9104

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Misook Kim et al.
Biochimica et biophysica acta, 1770(12), 1627-1635 (2007-10-09)
Two cysteine proteases, GP2 and GP3, have been isolated from ginger rhizomes (Zingiber officinale). GP2 is virtually identical to a previously identified ginger protease GPII [K.H. Choi, and R.A. Laursen, Amino-acid sequence and glycan structures of cysteine proteases with proline
E Okuno et al.
Biochimica et biophysica acta, 841(1), 112-119 (1985-07-26)
Quinolinic acid phosphoribosyltransferase (EC 2.4.2.19) was purified 3600-fold from rat liver and 280-fold from rat brain. Kinetic analyses (Km = 12 microM for the substrate quinolinic acid and Km = 23 microM for the cosubstrate phosphoribosylpyrophosphate), physicochemical properties of the
M A Zaval'nyĭ et al.
Voprosy virusologii, (5)(5), 583-589 (1980-09-01)
Primary cells of chick embryos (CEC), new-born piglet kidneys (NPK), and green monkey kidney (GMK) obtained by the conventional method of tissue trypsinization were shown to grow satisfactorily on microcarriers of various types. CEC formed a confluent monolayer on Supperbead
P K Farmer et al.
Biochimica et biophysica acta, 1434(1), 6-17 (1999-11-11)
A calmodulin-like domain protein kinase (DcCPK1, previously designated CDPK431) cloned from carrot (Daucus carota L.) was expressed at high levels in Escherichia coli and partially purified. Ca(2+)-induced gel mobility shift and (45)Ca(2+) ligand binding assays confirmed that recombinant DcCPK1 binds
Clovis R Nakaie et al.
Analytical biochemistry, 318(1), 39-46 (2003-06-05)
This report demonstrates that due to the presence of residual reactive sites in their matrices, classical diethylaminoethyl-attaching commercial anion-exchanger resins such as DEAE-MacroPrep and DEAE-Sephadex A50 supports can be used for peptide synthesis. Moreover, due to the high stability of
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Questions

1–5 of 5 Questions  

  1. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  2. How can Product A50120, DEAE-Sephadex® resin be regenerated?

    1 answer

    1. Regeneration is generally accomplished by washing with high ionic strength buffer or decreasing/increasing pH. The resin is then re-equilibrated in binding buffer.

      Helpful?

  3. What is the procedure for swelling Product A50120, DEAE-Sephadex® resin?

    1 answer

    1. Weigh out the required amount of dry powder and suspend it in the buffer of choice. Although the swelling volume will be specific to the binding buffer used, generally 1 gram of powder will swell to 25 mL final volume of medium. Excess binding buffer should be used. Swell the resin for 1-2 days at room temperature or 2 hours at 100°C over boiling water. After swelling, wash the resin on a Buchner funnel with binding buffer. Prepare a slurry with 75% swelled medium/25% binding buffer.

      Helpful?

  4. What procedures are used for elution when using Product A50120, DEAE-Sephadex® resin?

    1 answer

    1. For DEAE Sephadex, elution is achieved using either an increasing salt gradient or a decreasing pH gradient.

      Helpful?

  5. How can Product A50120, DEAE-Sephadex® resin, be stored?

    1 answer

    1. Store swollen medium refrigerated in the presence of a preservative such as 20% Ethanol. Sodium azide or thimerosal should not be used.

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